摘要
为研究日粮中添加不同比例黄金虫虫粉对动物肠道健康及局部炎症的影响,选取昆明小鼠28只,随机分为4组,各组基础日粮分别添加0%,5%,10%,20%黄金虫虫粉,饲喂2周后,灌胃0.05 mL/g的1×10^(8)CFU/mL的沙门菌菌液建立腹泻模型,荧光定量PCR检测小鼠肠道不同部位炎性因子及抗菌肽的表达量。结果显示,灌胃细菌后,饲喂不同比例黄金虫虫粉小鼠的空肠、回肠、结肠内的促炎因子TNF-α、IL-6表达量与对照组相比均明显下降,抗炎因子IL-4、IL-10和IL-13表达量明显升高,鼠源抗菌肽Ang-4、Cryptdin-1、Cryptdin-5、Lyz2、Crs1c和Reg3r表达量明显升高;这表明黄金虫虫粉作为饲料添加物,可以提高小鼠的免疫力,在沙门菌侵害肠道时,能增加肠道对病原菌的抵抗力以及提高机体抵御炎症反应的作用。
To study the effects of adding different proportions of the black soldier fly powder to the diet on the intestinal health and local inflammation of animals,28 Kunming mice were randomly divided into 4 groups,the basic diet of each group was supplemented with 0%,5%,10%,and 20%of black soldier fly powder.Two weeks later,1×10^(8)CFU of Salmonella was administered orally to establish a diarrhea model,and the expression of inflammatory factors and antibacterial peptides in different parts of the mouse intestine were detected by qRT-PCR.The results showed that after gavage,the expressions of proinflammatory factors TNF-αand IL-6 in the jejunum,ileum and colon of mice fed with different proportions of black soldier fly powder were significantly lower than those in the control group,the expression of anti-inflammatory factors IL-4,IL-10,and IL-13 were significantly increased,and the expression of mouse-derived antibacterial peptides Ang-4,Cryptdin-1,Cryptdin-5,Lyz2,Crs1 c,and Reg3 r were significantly increased too.These results indicated that the black soldier fly powder as a feed additive can improve the immunity of mice,and increase the resistance of intestine to pathogens and inhibit the inflammatory response when Salmonella invades the intestine.
作者
金鑫鑫
巩宇红
袁渤雨
刘明明
吴文湘
陈纯
苏铭信
王玮
JIN Xinxin;GONG Yuhong;YUAN BoyuA;LIU Mingming;WU Wenxiang;CHEN Chun;SU Mingxin;WANG Wei(College of Veterinary Medicine,Jilin University,Changchun 130062,China;Health Breeding Innovation Institute,College of Animal Science,Zhongkai University of Agriculture and Engineering,Guangzhou 510225,China)
出处
《中国兽医学报》
CAS
CSCD
北大核心
2021年第1期150-156,共7页
Chinese Journal of Veterinary Science
基金
国家自然科学基金资助项目(31872442)。
关键词
小鼠
黄金虫虫粉
沙门菌
抗菌肽
免疫力
肠道炎症
mice
black soldier fly powder
Salmonella
antibacterial peptides
immunity
intestinal inflammation
