摘要
目的采用UPLC-Q-Exactive四级杆-静电场轨道阱高分辨质谱联用技术,快速分析鉴定瑶药"十八钻"之一黄红钻的成分。方法采用Thermo Gold C18(2.1 mm×100 mm,1.9μm)色谱柱,乙腈(A)-0.1%甲酸水溶液(含10 mmol乙酸铵)(B)-为流动相梯度洗脱,流速0.3 mL·min-1,柱温35℃,正、负离子模式扫描。结果共鉴定出黄红钻中23个成分。包括有机酸类8个、生物碱类3个、木脂素类3个、环烯醚萜苷类5个。结论UPLC-Q-Exactive四极杆-静电场轨道阱高分辨质谱联用技术可以快速鉴定黄红钻化学成分,为黄红钻药效物质基础提供科学依据。
Objective To identify the chemical constituents of Jasminum pentaneurum Hand.-Mazz, one of the 18 zuan Yao medicines, by using ultra-high performance liquid chromatography coupled with hybrid quadrupole-orbitrap(UPLC-Q-Exactive) mass spectrometry method. Methods The analysis was conducted on a Thermo Gold C18(2.1 mm ×100 mm, 1.9 μm) by gradient elution. The mobile phase consisted of acetonitrile(A) and 0.1% aqueous solution of formic acid including 10 mmol ammomium acetate(B) was used, and flow rate was set at 0.3 mL/min. The column temperature was at 35℃. The MS information of the constituents was obtained in positive and negative modes.Results The research identified 23 constituents, including 8 organic acid, 3 alkaloids, 3 lignans and 5 iridoid glycosides. Conclusion Chemical constituents of Jasminum pentaneurum Hand.-Mazz could be fast identified by using UPLC-Q-Exactive method, which provides the scientific basis for the study of therapeutic efficacy of this plant.
作者
韦玮
张萌
周江煜
郝二伟
谢安然
陆海兰
王爱玲
梁琼平
侯小涛
邓家刚
Wei Wei;Zhang Meng;Zhou Jiangyu;Hao Erwei;Xian Anran;Lu Hailan;Wang Ailing;Liang Qiongping;Hou Xiaotao;Deng Jiagang(Guangxi Key Laboratory of Efficacy Study on Chinese Materia Medica,Nanning 530200,China;Collaborative Innovation Center of Study on Functional Ingredients of Agricultural Residues,Nanning 530200,China;School of pharmacy,Guangxi University of Chinese Medicine,Nanning 530200,China;Jinxiu Traditional Yao Medicine Hospital,Jinxiu 545700,China)
出处
《世界科学技术-中医药现代化》
CSCD
北大核心
2020年第11期3928-3935,共8页
Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology
基金
2017年广西中医药大学引进博士科研启动基金项目(2017BS044):黄红钻抗肿瘤药效评价、活性成分及其作用机制初步研究,负责人:韦玮
广西壮族自治区科学技术厅广西重点研发计划(桂科AB17195019):钻类瑶药抗肿瘤活性筛选与应用技术研究,负责人:郝二伟
广西壮族自治区科学技术厅广西科技基地和人才专项(桂AD19110155):中国-东盟传统药物研究国际合作联合实验室建设项目(二期),负责人:侯小涛。
