摘要
在山东青岛新发现一种蓝莓根腐病害,症状最初出现在根部,病根腐烂坏死,根状茎维管束和皮层变黑,导致根数量减少,叶片变黄、变红,植株生长不良,严重时叶片全部脱落,植株死亡。通过组织分离法和菌丝段分离法获得纯化菌株HMQAU180018,经柯赫氏法则验证,综合形态学特征及ITS区和cox I基因序列分析,菌株HMQAU 180018被鉴定为畸雌腐霉Pythium irregulare。这是国内首次描述畸雌腐霉引起蓝莓根腐病的报道。
Blueberry root rot disease was found for the first time in Qingdao,Shandong Province.The earliest symptoms occurred on root,then the diseased roots became rot and necrotic,and the vascular bundle and bark of rhizomes turned black.As a result,the radication became underdeveloped,the leaves turned yellow and red,and the plant grew poorly.In severe cases,all the leaves fell and the plant died.The pure isolate HMQAU180018 was obtained by tissue isolation method and mycelium segment isolation method.According to the pathogenicity test by Koch’s postulates,morphological and biological characteristics,sequence analyses of ITS and cox I,the pathogen was identified as Pythium irregulare.This is the first report of blueberry root rot caused by P.irregulare in China.
作者
周亚男
王博
刘婧
李海珍
刘爱娜
王连刚
李晓东
梁晨
ZHOU Ya-Nan;WANG Bo;LIU Jing;LI Hai-Zhen;LIU Ai-Na;WANG Lian-Gang;LI Xiao-Dong;LIANG Chen(Key Laboratory of Integrated Crop Pest Management of Shandong Province,College of Plant Health and Medicine,Qingdao Agricultural University,Qingdao,Shandong 266109,China;Qingdao West Coast Nonggao Science and Technology Service Co.,Ltd.,Qingdao,Shandong 266406,China;Qingdao Agricultural Machinery Service Center(Qingdao Agricultural Technology Extension Center),Qingdao,Shandong 266071,China)
出处
《菌物学报》
CAS
CSCD
北大核心
2020年第12期2261-2267,共7页
Mycosystema
基金
青岛市科技局项目(16-6-2-36-nsh)
国家重点研发计划项目(2017YFD0201104)。
关键词
畸雌腐霉
形态学
分子鉴定
蓝莓根腐病
Pythium irregulare
morphology
molecular identification
blueberry root rot
