摘要
为了更清楚地了解斑玉蕈菌丝成熟、原基形成和子实体发育的过程,本研究对不同菌丝培养时期析明的,:栽以斑玉培期瓶揭蕈进示菌行信丝出息培养菇素实信40验号-8,通0d并路对基过其因程不参中同与,培调子养节实时斑体期玉产和蕈量生菌呈长丝上发的升育生的关长趋键、势时子,期实说的体明x信形菌息成丝k素和的通发成路育熟?基的程因作度进用对行。产差研量异究会表结产达果生分表重要影响。对斑玉蕈基因组中的信息素信号通路基因进行分析鉴定共获得了8个关键基因。信息素通路基因差异表达分析表明:在菌丝培养40-80d过程中,大部分信息素信号通路基因在第60天时表达量最高,其中ste20、cdc24和ste12上调了4-20倍,而在第80天出现下降。从菌丝恢复到扭结形成原基和子实体发育的过程中,大多数基因在原基时期表达量最高,其中ste20、cdc24、ste11和ste12表达量上调最为显著,在子实体成熟期这些基因表达量下降。因此,这说明在菌丝营养生长过程中,在第60天菌丝细胞增殖生长最为旺盛,而在第80天菌丝细胞基本停止生长,菌丝也逐渐达到成熟。同时,在菌丝生殖生长过程中,斑玉蕈持续地上调信息素通路基因表达使菌丝细胞不断地分裂增殖,从而使新生的菌丝扭结形成原基,其中ste3、ste20、cdc24、ste11和ste12基因可能对斑玉蕈菌丝细胞的分裂增殖和诱导子实体形成起到关键的作用。
Fruiting experiment of different mycelial growth stages of Hypsizygus marmoreus in bottled cultivation was carried out,and differential expression of pheromone pathway genes in different cultivation stages and key growth stages was investigated for revealing the role of pheromone signaling pathway genes in regulating the mycelial maturation,primordia formation and fruiting body development of the fungus.The results show that during 40–80 day mycelial cultivation,the fruit body production trends to upward tendency,indicating that the mycelium maturity has an important impact on the production.A total of eight key genes was obtained by analyzing and identifying the pheromone signaling pathway genes in the H.marmoreus genome.The differential expression analysis of pheromone pathway genes showed that during 40–80 day mycelial culture,most of the pheromone signaling pathway genes had the highest expression levels on the 60 th day,of which ste20,cdc24,and ste12 were up-regulated 4–20 times,but decreased on the 80 th day.In the process of mycelial regeneration,primordium formation and fruiting body development,most of the genes had the highest expression in the primordium stage and the expression decreased in the fruiting stage,of which the expression levels of ste20,cdc24,ste11 and ste12 were most significantly up-regulated.This indicated that the mycelial cells proliferated most vigorously on the 60 th day,and began stagnancy on the 80 th day,and then the mycelia gradually matured.During reproductive growth,the fungus continuously up-regulates the pheromone pathway gene expression to multiply the mycelial cells for primordium formation,in which the genes ste3,ste20,cdc24,ste11 and ste12 might play key role in proliferation of mycelia and the formation of fruiting bodies.
作者
陈辉
郝海波
赵静
王倩
隽加香
陈明杰
冯志勇
张津京
CHEN Hui;HAO Hai-Bo;ZHAO Jing;WANG Qian;JUAN Jia-Xiang;CHEN Ming-Jie;FENG Zhi-Yong;ZHANG Jin-Jing(Institute of Edible Fungi,Shanghai Academy of Agricultural Sciences,Shanghai Key Laboratory of Agricultural Genetics and Breeding,Key Laboratory of Resources and Utilization of Edible Fungi(South),Ministry of Agriculture,National Engineering Research Center of Edible Fungi,Shanghai 201403,China;Shanghai Bright Esunyes Bio-tech Co.,Ltd.,Shanghai 201408,China;College of Life Sciences,Nanjing Agricultural University,Nanjing,Jiangsu 210014,China)
出处
《菌物学报》
CAS
CSCD
北大核心
2020年第12期2304-2313,共10页
Mycosystema
基金
国家自然科学基金(31601802)
上海市现代农业产业技术体系建设[沪农科产字(2020)第9号]。
关键词
斑玉蕈
信息素通路基因
细胞增殖
差异表达
Hypsizygus marmoreus
pheromone pathway gene
cell proliferation
differential expression
