摘要
目的探讨肿瘤转移相关因子1(MTA1)和血管内皮因子(VEGF)在口腔鳞状细胞癌(OSCC)组织中的表达及其与OSCC侵袭、转移的关系。方法采用实时定量聚合酶链式反应、免疫组织化学法检测MTA1、VEGF在OSCC癌灶、癌周边缘及癌周正常组织中的表达情况。结果 MTA1、VEGF在癌周正常组织和OSCC癌灶组织中的阳性表达率,以及MTA1 mRNA和VEGF mRNA在OSCC癌灶、癌周边缘及癌周正常组织中的相对表达量比较差异均有统计学意义(P<0.05或0.01),高、中、低分化OSCC的MTA1 mRNA相对表达量差异也有统计学意义(P<0.01),伴有颈淋巴结转移的MTA1的阳性表达率高于无颈淋巴结转移者(P<0.05)。MTA1和VEGF以及MTA1 mRNA和VEGF mRNA在OSCC组织中表达均呈正相关(r=0.588、0.826,P<0.05或0.01)。结论 MTA1在OSCC中的表达与其病理学分级及颈淋巴结转移有关,MTA1和VEGF可能在OSCC侵袭转移中起协同作用。
Objective To investigate the expression of metastasis-associate gene 1(MTA1) and vascular endothelial factor(VEGF) in oral squamous cell carcinoma(OSCC) and its correlation with OSCC invasion and metastasis. Methods Real-time quantitative polymerase chain reaction(RT-PCR) and immunohistochemistry(IHC) method were used to detect the expression of MTA1 and VEGF in OSCC tissues, adjacent tumor tissues and adjacent normal tissues of the patients with OSCC. Results There were statistical differences in the positive expression rates of MTA1 and VEGF in the adjacent normal tissues and in the OSCC tissues as well as relative expression of MTA1 mRNA and VEGF mRNA in OSCC tissues, adjacent tumor tissues and adjacent normal tissues(P<0.05 or 0.01). There was also statistical difference in the relative expression of MTA1 mRNA in the high, medium and low differentiated groups(P<0.01). The positive expression rate of MTA1 with cervical lymph node metastasis was significantly higher than that without cervical lymph node metastasis(P<0.05). The expression of MTA1 and VEGF as well as MTA1 mRNA and VEGF mRNA in OSCC tissues were positively correlated(r=0.588, 0.826,P<0.05 or 0.01). Conclusions The expression of MTA1 in OSCC is related to the pathological grading of OSCC and the metastasis of cervical lymph node. MTA1 and VEGF may play a synergistic role in the invasion and metastasis of OSCC.
作者
陈旭斌
陈坤
周昊
张铁柱
王敬雯
CHEN Xu-bin;CHEN Kun;ZHOU Hao;ZHANG Tie-zhu;WANG Jing-wen(Department of Oral and Maxillofacial Surgery,Hainan General Hospital,Haikou 570113,China;Department of Stomatology,the Affiliated Hospital of Guangdong Medical University,Zhanjiang 524001,China;Department of Stomatology,Wuhan Cancer Hospital,Wuhan 430014,China)
出处
《广东医科大学学报》
2021年第1期26-30,共5页
Journal of Guangdong Medical University
基金
湛江市科技计划项目(No.2013A01013)。
