摘要
采用人工模拟酸雨的实验设计,以国家农业地理标志产品香青菜(Brassica chinensis L.)为实验材料,以叶片喷施蒸馏水为对照(CK),设置直接喷施pH3.0的酸雨(SY)和10mg·L^(-1)浓度五酸钾溶液预处理后再喷施pH3.0的酸雨(WS)两个处理组。测定并比较分析各处理组香青菜根系活力、质膜透性(MP)、过氧化氢酶(CAT)活性、超氧阴离子自由基产生速率和脯氨酸(PRO)含量,研究酸雨胁迫对香青菜主要生理指标的影响和五酸钾对酸雨胁迫伤害香青菜的缓解效应,从而探索五酸钾对酸雨胁迫下植物幼苗的防护效果。结果表明,叶面喷施浓度10mg·L^(-1)的五酸钾溶液能够显著提高酸雨胁迫下香青菜根系活力、CAT活性,保护了细胞质膜,有效降低香青菜体内PRO含量及超氧阴离子自由基产生速率。说明五酸钾可缓解酸雨对香青菜的生理伤害,对提高香青菜抗酸雨伤害能力具有优良效果。
In this paper,the simulating acid rain(pH3.0)pollution was used,Brassica chinensis L.,a national agricultural geographical indication product,regarded as the model material.The leaves which were sprayed distilled water regard as the control(CK).One treatment is the leaves were sprayed of pH 3.0 artificial acid rain(SY).Another treatment is the leaves were sprayed with 10mg·L^(-1) Pyridine-2,3,4,5,6-pentacarboxylate solution and pH3.0 artificial acid rain(WS)in sequence.The root activity,plasma membrane permeability(MP),catalase(CAT)activity,oxygen free radical producing rate and proline(PRO)content were studied under the condition of acid rain stress and pyridine-2,3,4,5,6-pentacarboxylate protection.The results showed that the leaf which sprayed 10mg·L^(-1) pyridine-2,3,4,5,6-pentacarboxylate solution could significantly improve the root activity and CAT activity,protect the cytoplasm membrane,and effectively reduce the PRO content and oxygen free radical production rate in broccoli under acid rain stress.The comprehensive comparison shows that pyridine-2,3,4,5,6-pentacarboxylate have a positive effect on relieving the physiological damage under the acid rain stress and improving the ability of resisting acid rain.
作者
谢恩耀
卫慢
陈俊彤
朱志平
袁振兴
张颖
钱加越
金琎
XIE En-yao;WEI Man;CHEN Jun-tong;ZHU Zhi-ping;YUAN Zhen-xing;ZHANG Ying;JIN Jin(School of Chemical Biology and Materials Engineering,Suzhou University of Science and Technology,Suzhou 215009,China;Shanghai Weipu Chemical Technology Co.,LTD,Shanghai 201403)
出处
《中国农业气象》
CSCD
北大核心
2021年第3期213-220,共8页
Chinese Journal of Agrometeorology
基金
苏州市科技计划项目(SYN201413)
江苏省教育厅“高等学校大学生实践创新训练”(601630002)。
关键词
香青菜
酸雨胁迫
五酸钾
理化指标
化控减灾
Brassica chinensis L.
Acid rain stress
Pyridine-2,3,4,5,6-pentacarboxylate
Physical and chemical indexes
Chemical control and disaster reduction
