摘要
为探明白刺(Nitraria tangutorum)14-3-3基因家族的基本特征及其进化关系,本研究基于白刺转录组数据,利用生物信息学方法,对白刺14-3-3基因家族成员进行鉴定,预测其理化性质、亚细胞定位、保守结构域,分析其系统进化关系及其基因在不同浓度盐、干旱处理下的组织器官表达模式。结果表明:从白刺转录组数据中初步筛选出10个14-3-3家族成员,其中具有完整蛋白质编码区(Coding Sequence,CDS)的9个成员,其蛋白的编码氨基酸的数目为212~297个,等电点在4.73~6.14之间,分子量范围是24.06~33.51 kDa,二级结构主要以α螺旋为主,三级结构高度保守;亚细胞定位预测显示该家族成员主要分布在细胞质上;表达模式分析表明,14-3-3基因在白刺不同组织器官中呈现不同的表达规律,且参与应答外界的干旱和盐胁迫。由此推测,14-3-3基因家族可能在白刺的胁迫防御方面发挥重要功能。本研究结果为进一步深入揭示白刺14-3-3基因家族的功能奠定基础。
To explore the basic characteristics and evolutionary relationship of the 14-3-3 gene family in Nitraria tangutorum,based on the transcriptome data,we used the bioinformatics methods identified the members of the 14-3-3 gene family of N.tangutorum,predicted their physicochemical property,subcellular localization,conserved domains,and analyzed their phylogenitic relationships,and expression patterns in different tissues and organs under different concentrations of salt and drought stresses.The results showed that ten 14-3-3 family members were preliminarily screened from the transcriptome data of N.tangutorum.Among them,9 members with complete protein(coding sequence CDS)had 212~297 amino acids,the isoelectric point was 4.73~6.14,and the molecular weight range was 24.06~33.51 kDa,the secondary structure was mainlyα-helix,and the tertiary structure was highly conservative.Subcellular localization prediction showed that the family members were mainly distributed in the cytoplasm.The expression pattern analyses showed that 14-3-3 genes have different expression patterns in different tissues and organs of N.tangutorum,and are participated in response to drought and salt stresses.Therefore,the 14-3-3 gene family may play an important role in stress defense of N.tangutorum.The results of this investigation could provide an foundation for further reveal the function of the 14-3-3 gene family in N.tangutorum.
作者
易丹
王博
段慧荣
李毅
王丽蓉
YI Dan;WANG Bo;DUAN Hui-rong;LI Yi;WANG Li-rong(College of Forestry,Gansu Agricultural University,Lanzhou,Gansu Provicne 730070,China;Qinghai University for Nationalities,Xining,Qinghai Provicne 810000,China;Lanzhou Institute of Animal Husbandry and Veterinary Medicine,Chinese Academy of Agricultural Sciences,Lanzhou,Gansu Provicne 730050,China)
出处
《草地学报》
CAS
CSCD
北大核心
2021年第3期443-456,共14页
Acta Agrestia Sinica
基金
青海民族大学校级高层次人才项目(No.2018XJG07)
国家自然科学基金(31860118)
甘肃省林业科技创新与国际合作资金(GLC2019-418-8)
“丝绸之路”草原生态修复技术模式研究与示范,对发展中国家常规性科技援助项目(KY202002011)
中央财政林业科技推广示范资金和甘肃省科技创新基地与人才计划(17 JR7 WA018)资助。
