摘要
[目的]探讨珍稀濒危植物金丝李种子休眠解除的分子机理,挖掘其休眠解除相关的候选基因,以期为金丝李的育种、保护和利用提供有益参考。[方法]以新鲜成熟种子(OS)、播种60 d未萌发的种子(UG)及播种60 d已萌发的种子(GS)的胚和胚乳为材料进行转录组测序。[结果](1)从3组样本中得到120040个unigenes,其中,56224个unigenes在KEGG数据库中获得注释;(2)GS相对OS有1174个unigenes上调表达,2232个下调,UG相对OS有849个unigenes上调,1926个下调,GS相对UG有814个unigenes上调,670个下调;(3)筛选出差异量较大的122个基因中,显著上调的基因涉及钙信号、次生代谢产物合成、核糖体和植物激素信号转导等通路,显著下调的基因涉及糖酵解/糖异生、脂肪酸合成与降解、内质网中的蛋白质加工和氨基酸合成等通路;(4)筛选出40个涉及到与ABA、GA、生长素和乙烯等激素相关的DEGs,其中,ZEP、GA2ox1、GA2ox3、GAI、GID1、SnRK2.2和ACO1在种子休眠解除时显著下调表达,而CYP707A2、GA20ox1和GA3ox2显著上调表达。[结论]推测金丝李种子休眠解除过程中,通过上调钙信号、次生代谢产物合成等通路,下调糖酵解/糖异生、脂肪酸合成与降解等通路,同时伴随着上调GA合成及ABA分解的相关基因,下调ABA合成及GA分解的相关基因,与有关转录因子等调控网络相配合以打破休眠。
[Objective]The objective of this study is to provide references for breeding,protection and utilization of Garcinia paucinervis through studying the molecular mechanisms of dormancy release and gaining the candidate genes associated with dormancy release.[Method]Transcriptome sequencing was carried out on three groups of samples:fresh mature seeds(OS),the ungerminated seeds after sowing for 60 days(UG)and the germinated seeds after sowing for 60 days(GS).[Result](1)120040 unigenes were obtained from the three groups of samples,among which 56224 were annotated in KEGG databases.(2)Compared with the OS,1174 up-regulated unigenes and 2232 down-regulated unigenes were obtained in the GS.Compared with the OS,849 up-regulated unigenes and 1926 down-regulated unigenes were obtained in the UG.Compared with the UG,814 up-regulated unigenes and 670 down-regulated unigenes were obtained in the GS.(3)Among the 122 genes which were screened to be significantly differentially expressed,the significantly up-regulated genes involved pathways including calcium signal,biosynthesis of secondary metabolites,ribosome and plant hormone signal transduction,while the significantly down-regulated genes involved pathways including glycolysis/gluconeogenesis,fatty acid biosynthesis and degradation,protein processing in endoplasmic reticulum and biosynthesis of amino acids.(4)Forty DEGs were screened involving ABA,GA,auxin,ethylene and other hormones,among which ZEP,GA2ox1,GA2ox3,GAI,GID1,SnRK2.2,and ACO1 were significantly down-regulated during the dormancy release of seeds,while CYP707A2,GA20ox1,and GA3ox2 were significantly up-regulated.[Conclusion]The dormancy of G.paucinervis seeds might be broken by up-regulating pathways including calcium signal and biosynthesis of secondary metabolites,and down-regulating pathways including glycolysis/gluconeogenesis,fatty acid biosynthesis and degradation,etc.,followed by up-regulating of related genes of GA synthesis and ABA decomposition,coupled with down-regulating of related genes of ABA synthesis and GA decomposition to interact with the regulatory networks of transcription factors during the dormancy release of G.paucinervis seeds.
作者
张俊杰
张灵艺
夏科
蒋冕
韦霄
ZHANG Jun-jie;ZHANG Ling-yi;XIA Ke;JIANG Mian;WEI Xiao(School of Architecture and Urban Planning,Chongqing Jiaotong University,Chongqing 400074,China;Guangxi Institute of Botany,Chinese Academy of Sciences,Guilin 541006,Guangxi,China;College of Plant Protection,Hunan Agricultural University,Changsha 410004,Hu’nan,China)
出处
《林业科学研究》
CSCD
北大核心
2021年第1期35-46,共12页
Forest Research
基金
广西自然科学基金项目(2015GXNSFDA13915)
广西科技基地和人才专项(桂科AD17129022)
重庆交通大学博士科研启动项目(2020019089)。
