摘要
[目的]为了解红树植物根部内生微生物的固氮功能和获得固氮能力较高的固氮菌,本实验从红树植物-桐花树根部组织分离纯化得到具有固氮能力的菌株。[方法]以Ashby无氮培养基为选择分离条件,从表面消毒的植物根部组织分离得到内生固氮菌,并通过凯氏定氮法检测菌株的固氮能力;采用单因素法优化培养条件,获得菌株典型生长曲线。[结果]实验筛选得到固氮能力较高的一株固氮菌A1,从形态学特征等方面进行研究,为革兰氏阴性菌,菌落圆形乳白色,菌株固氮量为16.695 mg·L^(−1),最适生长条件:温度32℃,pH值7,NaCl浓度2%,最适碳源淀粉。[结论]本实验为培育优良的红树植物内生固氮菌与研制高效固氮菌剂应用于生产实践提供理论依据。
[Objective]To understand the nitrogen fixation function of the endophytic microorganisms in the roots of mangrove plants and obtain nitrogen-fixing bacteria with higher nitrogen fixation ability by isolating the nitrogen-fixing strains and purifying them from root tissues of mangrove plants.[Method]The Ashby nitrogen-free medium was selected as separation condition,and the endophytic nitrogen-fixing bacteria were isolated from surface of surfacesterilized plant roots,then the nitrogen-fixing ability of the strain was determined by Kjeldahl method.The monofactor method was used to optimize the culture conditions,and typical growth curve of strain was obtained.[Result]The results showed that a strain of nitrogen-fixing bacterium A1 with high nitrogen-fixing ability was selected as Gram-negative bacterium,and its colony was round milky white,the nitrogen fixation rate of the strain was 16.695 mg·L^(−1).Its optimum growth conditions were temperature 32°C,pH 7,NaCl concentration 2%,and the optimum carbon source was starch.[Conclusion]This experiment can provide a theoretical reference for the cultivation of excellent mangrove nitrogen-fixing bacteria and the development of high-efficiency nitrogen-fixing bacteria.
作者
蔺红苹
谢呈媛
王芸
成夏岚
LIN Hong-ping;XIE Cheng-yuan;WANG Yun;CHENG Xia-lan(School of Life Science and Technology,Lingnan Normal University,Zhanjiang 524048,Guangdong,China)
出处
《林业科学研究》
CSCD
北大核心
2021年第1期181-186,共6页
Forest Research
基金
岭南师范学院实验室安全建设与管理专项项目
国家星火计划2012GA780021项目资助。
关键词
内生固氮菌
筛选
培养特性
endophytic nitrogen fixation bacteria
screen
culture characteristics
