摘要
目的:建立高效液相色谱-二极管阵列检测器法(HPLC-DAD)同时测定风热感冒颗粒中苦杏仁苷、(R,S)-告依春、芦丁、连翘酯苷A、3,5-O-二咖啡酰基奎宁酸和牛蒡苷含量的方法。方法:采用液相色谱法,色谱柱为Waters Symmetry ShieldTM RP18,乙腈为流动相A,0.1%磷酸水溶液为流动相B,梯度洗脱;流速为1.0 mL·min-1;柱温为30℃;检测波长为苦杏仁苷和牛蒡苷207 nm,(R,S)-告依春245 nm,芦丁、连翘酯苷A和3,5-O-二咖啡酰基奎宁酸330 nm。结果:苦杏仁苷、(R,S)-告依春、芦丁、连翘酯苷A、3,5-O-二咖啡酰基奎宁酸和牛蒡苷的线性范围分别为23~920(r=0.9994)、0.4~16(r=0.9995)、4.4~176(r=0.9999)、2.8~110(r=0.9998)、5.4~216(r=0.9996)、38~1520μg·mL^(-1)(r=0.9994),加样回收率的平均值(n=6)分别为98.8%、98.7%、98.4%、99.2%、99.8%和98.7%,RSD分别为1.0%、2.5%、1.8%、1.3%、1.3%和0.8%。结论:本法操作简便、结果准确、重复性好,可用于风热感冒颗粒的质量控制。
Objective:To establish an HPLC-DADmethod for determination of amygdalin,(R,S)-epigoitrin,rutin,forsythiaside A,3,5-O-dicaffeoyl quinic acid and arctiin in Fengreganmao granules.Methods:A stable HPLC method was established and the chromatography was accomplished on a Waters Symmetry ShieldTM RP18 column,the mobile phase consisted of acetonitrile(A)-0.1%(w)phosphoric acid(B)as mobile phase in a gradient mode.The flow rate was 1.0 mL·min-1 and the column temperature was set at 30℃.The detection wavelength was set at 207 nm for amygdalin and arctiin,245 nm for(R,S)-epigoitrin,330 nm for rutin,forsythiaside A and 3,5-O-dicaffeoyl quinic acid,respectively.Results:The linear ranges of amygdalin,(R,S)-epigoitrin,rutin,forsythiaside A,3,5-O-dicaffeoyl quinic acid and arctiin were 23-920(r=0.9994),0.4-16(r=0.9995),4.4-176(r=0.9999),2.8-110(r=0.9998),5.4-216(r=0.9996)and 38-1520μg·mL^(-1)(r=0.9994),respectively.The average recovery were 98.8%,98.7%,98.4%,99.2%,99.8%and 98.7%,RSD were 1.0%,2.5%,1.8%,1.3%,1.3%and 0.8%.Conclusion:The method is simple to operate,accurate and reproducible,which can be used for the quality control of Fengreganmao granules.
作者
刘斌
LIU Bin(Taian Testing Institute for Food and Drug Control,Taian 271000,China)
出处
《中国现代中药》
CAS
2021年第1期147-152,共6页
Modern Chinese Medicine
基金
2019—2020年度山东省中医药科技发展计划项目(2019-0768)。
