摘要
采用GB 5009.93—2017《食品安全国家标准食品中硒的测定》中规定的食品中硒元素含量检测方法对食品中硒元素进行含量检测,所得标准曲线方程为y=2626.595x+126.650,R^(2)=1.000,硒元素在0~100μg/L范围内线性相关系数均为1.000,线性关系良好,并通过样品验证证明该方法准确有效。另外,该试验建立了利用高效液相色谱-电感耦合等离子色谱(HPLC-ICP-MS)联用分离检测富硒食品中硒酸根(SeO_(4)^(2-))、亚硒酸根(SeO_(3)^(2-))、硒代胱氨酸(SeCys_(2))、硒甲基硒代半胱氨酸(MeSeCys)、硒代蛋氨酸(SeMet)5种不同硒元素形态的方法。该方法利用胃蛋白酶在0.1%盐酸体系下酶解提取食品中的硒化合物,采用Hamilton PRP X-100阴离子交换柱作为分离柱,以5 mmol/L柠檬酸、氨水调节pH 4.7为流动相,等度洗脱分离5种硒化物,再运用ICP-MS进行检测。结果表明,该方法对于5种硒元素形态分离效果较好,在900 s内可完成5种硒形态的快速准确分析,是一种有效可行的对富硒产品中硒形态的分析方法。
This study uses GB 5009.93—2017"National Food Safety Standard-Determination of Selenium in Foods"to determine the content of selenium in food.The obtained standard curve equation is y=2626.595 x+126.650,R^(2)=1.000,the linear correlation coefficients of selenium in the range of 0-100μg/L are all 1.000,and the linear relationship is good.The method is proved to be accurate and effective by sample verification.In addition,a method for the determination of 5 selenium species(SeO_(4)^(2-),SeO_(3)^(2-),SeCys_(2),MeSeCys and and SeMet)in selenium-enriched foods by high performance liquid chromatography-inductively coupled plasma chromatography(HPLC-ICP-MS)has been established.This method uses pepsin to extraction of selenium compounds in food under 0.1%hydrochloric acid system.A Hamilton PRP X-100 anion exchange column is used as the separation column,5 selenium compounds are eluted and separated with 5 mmol/L citric acid and ammonia water to adjust the pH to 4.7,and then detected by ICP-MS.The results show that this method has a good effect on the separation of 5 selenium element forms.The fast and accurate analysis of five selenium forms within 900 s.It is an effective and feasible analysis method for selenium forms in selenium-rich products.
作者
黄韬睿
王小平
王鑫
HUANG Taorui;WANG Xiaoping;WANG Xin(Sichuan University of Tourism,Chengdu 610100;Sichuan Institute for Food and Drug Control,Chengdu 610097)
出处
《食品工业》
CAS
2021年第2期311-315,共5页
The Food Industry
基金
四川省教育厅自然科学重点项目(17ZA0293)
川菜发展研究中心项目(CC20Z17)
干热河谷特色生物资源开发四川省高校重点实验室项目(GR-2020-C01)。
关键词
高效液相色谱-电感耦合等离子质谱
富硒食品
硒的形态
high performance liquid chromatography-inductively coupled plasma mass spectrometry
selenium-enriched foods
speciation of selenium
