摘要
采用傅里叶变换红外光谱法和显微红外光谱技术对不同产地桔梗进行鉴别。结果表明,不同产地桔梗的一阶红外光谱图整体上差别并不明显,二阶导数处理后,各产地的特征峰差异得以显现,显微红外光谱图则进一步直观清晰地显现出桔梗皂苷d的含量多少与分布区域,进一步佐证了不同产地桔梗间桔梗皂苷d的含量差异的结果。根据不同产地地理远近来分析,不同省之间的桔梗的物质含量与分布的差距比较明显;同省不同市之间几乎没有太大的差距,可能原因是同省不同市的地理距离太小。因此,红外光谱法和显微红外光谱技术提供了大量桔梗的整体化学成分信息,递进式地验证了不同产地桔梗之间的所含物质的量及分布有差异,可以初步鉴别不同产地桔梗,为今后系统完整的选择桔梗入药产地提供依据。
Fourier transform infrared spectroscopy(FT-IR) and Fourier transform infrared micro spectroscopy(Micro-FTIR) were used for the identification of Platycodon grandiflorum from different origins.The difference in the FT-IR spectra of Platycodon grandiflorum from different origins was not obvious.After the secondary derivative infrared spectra(SD-IR),the difference of the characteristic peaks of each origin can be revealed.Moreover,Micro-FTIR spectroscopy visually and clearly showed the difference of content and distribution area of platycod on from different producing areas,which further testified the differences in the content of platycodin d from different origins.Based on the analysis of the geographical distance of different origins,the difference in the content and distribution of Platycodon grandiflorum between different provinces was obvious.However,there was almost no big gap between different cities in the same province,which may be due to the close geographical distance of different cities in the same province.A lot information of Platycodon grandiflorum can be provided by FT-IR spectra and Micro-FTIR spectroscopy which can testify that the content and structure of substance of Platycodon grandiflorum from different origins were different.The method can also be used for analysis and identification of Platycodon grandiflorum from different sources which can make a good foundation for further research.
作者
司雨柔
高韵
解玫莹
李欢欢
程旺兴
SI Yu-rou;GAO Yun;XIE Mei-ying;LI Huan-huan;CHENG Wang-xing(Anhui Engineering Technology Research Center of Modern Chinese Medicine,School of Pharmacy,Anhui University of Chinese Medicine,Hefei 230012,China)
出处
《化学试剂》
CAS
北大核心
2021年第2期210-215,共6页
Chemical Reagents
基金
安徽高校自然科学研究项目(KJ2018A0300)
安徽省重点研究与开发计划项目(201904a07020073)
国家重点研发计划项目(2017YFC1701601)。
关键词
桔梗
傅里叶红外光谱技术
二阶导数谱
显微红外光谱技术
Platycodon grandiflorum
Fourier transform infrared spectroscopy
second derivative infrared spectroscopy
Fourier transform infrared micro spectroscopy
