摘要
该文建立UHPLC方法测定参附注射液中单酯型生物碱的含量。采用Agilent Zorbax Eclipse Plus C18(2.1 m×100 mm,1.8μm)色谱柱;流动相为甲醇∶0.01 mol/L乙酸铵溶液(冰乙酸调pH=5.0)(39∶61);流量为0.4 mL/min;柱温为30℃;检测波长为235 nm。结果表明苯甲酰新乌头原碱的进样量在0.90-28.78 ng范围内线性关系良好(R=0.9998),平均回收率为99.9%(RSD为1.98%);苯甲酰乌头原碱的进样量在0.86-27.56 ng范围内线性关系良好(R=0.9998),平均回收率为99.4%(RSD为1.64%);苯甲酰次乌头原碱的进样量在0.84-26.95 ng范围内线性关系良好(R=0.9998),平均回收率为103.3%(RSD为1.29%)。该方法快速、简便、准确度高、重复性好,可用于参附注射液中单酯型生物碱的含量测定。
To establish a UHPLC method for determining monoester alkaloids in Shenfu injection.The column of Agilent Zorbax Eclipse Plus C18(2.1 m×100 mm,1.8μm)was used;the mobile phase consisted of methanol∶0.01 mol/L ammonium acetate solution(pH=5.0 adjusted by glacial acetic acid)(39∶61);the flow rate was 0.4 mL/min;the column temperature was 30℃;the detection wavelength was 235 nm.The linearity of benzoylneoaconitine was good in the range of 0.90–28.78 ng(R=0.9998),and the average recovery was 99.9%(RSD:1.98%);the linearity of benzoylaconitine was good in the range of 0.86–27.56 ng(R=0.9998),the average recovery was 99.4%(RSD:1.64%);the linearity of benzoylhypoaconitine was good in the range of 0.84–26.95 ng(R=0.9998),the average recovery was 103.3%(RSD:1.29%).The method is rapid,simple,accurate and reproducible,and suitable for the determination of monoester alkaloids in Shenfu injection.
作者
袁海英
蔡帮军
YUAN Haiying;CAI Bangjun(China Resources Sanjiu(Ya’an)harmaceutical Co.,Ltd.,Ya’an 625000,China)
出处
《中国测试》
CAS
北大核心
2021年第3期59-63,共5页
China Measurement & Test
基金
国家中医药管理局中药标准化建设项目(ZYBZH-C-SC-51)。
