摘要
【目的】研究掌握云南省规模化猪场副猪嗜血杆菌的流行血清型及敏感药物,为该省副猪嗜血杆菌防控提供依据。【方法】应用TSA培养基从云南规模化养猪场疑似副猪嗜血杆菌病例样品中分离到2株细菌(DYJ20160901、CH20190501),观察细菌形态学、进行生化试验、观察卫星生长现象、进行特异性片段PCR扩增和药敏试验,以及对菌株进行mPCR分子血清定型和16S rRNA序列分析。【结果】2菌株培养特性、形态学特性及生化试验与副猪嗜血杆菌相似,且均扩增出了1090 bp的特异性片段;通过mPCR分子血清定型在5/12型泳道扩增出了450 bp的特异性片段,通过16S rRNA序列分析显示2菌株与Genbank中已公布的不同血清型HPS菌株同源性为97.3%~99.6%,分别与Genbank登录号FJ667952、AB078972的两株血清5型的菌株构成一个分支,同源性高达99.1%和99.6%。2菌株药敏试验高度敏感率达100%的药物:强力霉素、大观霉素、头孢噻肟、氟苯尼考、头孢曲松钠,耐药率为100%的药物:林可霉素。【结论】DYJ20160901、CH20190501菌株均为副猪嗜血杆菌血清5型;二者都高度敏感的药物是强力霉素、大观霉素、头孢噻肟、氟苯尼考、头孢曲松钠。
【Objective】The aim of study is to understand the epidemic serotypes and sensitive drugs of Haemophilus parasuis in large-scale pig farms and provide evidences for the prevention and control of Haemophilus parasuis in Yunnan Province.【Method】Two strains of bacteria(DYJ20160901,CH20190501)were isolated from suspected H.parasuis cases in large-scale pig farms by using TSA medium.Morphological observation,biochemical tests,satellite growth phenomena,and specific fragment PCR amplification were performed on the bacteria.Moreover,the drug sensitivity test was also carried out,and the mPCR molecular serotyping and 16 S rRNA sequence analysis were performed on DYJ20160901 and CH20190501 strains.【Result】The cultural characteristics,morphological characteristics and biochemical tests of the two strains were similar to those of Haemophilus parasuis.The specific 1090 bp fragments were amplified in all of the strains.The 450 bp specific fragments were amplified by using mPCR molecular serotyping in DYJ20160901 and CH20190501 strains.The 16 S rRNA sequence analysis showed 97.3%-99.6%homology of the two strains with the published HPS serotypes in Genbank,which formed a branch with the FJ667952 and AB078972 strains;The following drug got the sensitivity test of two strains with 100%sensitivity rate:doxycycline,spectinomycin,cefotaxime,florfenicol and ceftriaxone sodium.And the following drugs got the 100%resistance rate:lincomycin.【Conclusion】All the two strains belong to Haemophilus parasuis type V.They are highly sensitive to doxycycline,spectinomycin,cefotaxime,florfenicol,ceftriaxone sodium.
作者
赵津
吴林蔚
李珂
赵国洪
杨锁柱
王洪伟
韩建强
ZHAO Jin;WU Lin-wei;LI Ke;ZHAO Guo-hong;YANG Suo-zhu;WANG Hong-wei;HAN Jian-qiang(Yuxi Agricultural Vocation-Technical Collge,Yunnan Yuxi 653106,China;Genter for Disease Control and Prevention of Yuxi Gity,Yunnan Yuxi 653100,China;Yunnan Animal Science and Veterinary Instiute,Yunnan Kunming 650224,China)
出处
《西南农业学报》
CSCD
北大核心
2021年第2期412-420,共9页
Southwest China Journal of Agricultural Sciences
基金
云南省教育厅科学研究基金项目(2016ZZX288)。
关键词
云南省
副猪嗜血杆菌
分离
鉴定
mPCR分子血清分型
药敏试验
Yunnan Province
Haemophilus parasuis
Isolation
Identification
mPCR molecular serotyping
Drug sensitivity test
