摘要
目的探究安徽生产的多花黄精多糖3,5-异构酶/4-还原酶基因(PcUER1)的结构基因信息及其在多花黄精中单糖鼠李糖化合物生源路径中的表达调控机制。方法以多花黄精为研究对象,基于其转录组学数据,克隆PcUER1基因并针对该cDNA序列展开生物信息学分析,采用实时荧光定量PCR方法检测多花黄精不同组织中PcUER1基因的表达水平。结果多花黄精多糖PcUER1基因的cDNA序列长度为900 bp,编码299个氨基酸;序列分析表明多花黄精与百合科虎眼万年青的相似度达到92.03%;实时荧光定量PCR检测结果显示,PcUER1基因在多花黄精的根、须根、茎、叶中表达水平逐渐降低,茎和叶中PcUER1基因表达水平的差异无统计学意义。结论多花黄精多糖PcUER1基因的活性位点有辅因子结合基序和催化四分体基序,其蛋白表达产物理化性质稳定。
Objective To investigate the structural and genetic information of the 5-isomerase/4-reductase gene(PcUER1)of Polygonatum cyrtonema Hua produced in Anhui,China and its expression and regulation mechanism in the biogenic pathway of the monosaccharide rhamnose compounds in Polygonatum cyrtonema Hua.Methods Based on the transcriptome data of Polygonatum cyrtonema Hua,the PcUER1gene was cloned and a bioinformatics analysis was performed for the cDNA sequence.Quantitative real-time PCR was used to measure the mRNA expression of PcUER1 in different tissues of Polygonatum cyrtonema Hua.Results The cDNA sequence of the PcUER1 gene in Polygonatum cyrtonema Hua was 900 bp in length and encoded 299 amino acids.Sequence analysis showed that Polygonatum cyrtonema Hua had high homology with Ornithogalum caudatum in Liliaceae,with a rate of 92.03%.Quantitative real-time PCR showed the highest mRNA expression of PcUER1 in the root of Polygonatum cyrtonema Hua,followed by fibrous root,stem,and leaf,and there was no significant difference in the mRNA expression of PcUER1 between the leaf and the stem.Conclusion The active site of the PCUER1 gene in Polygonatum cyrtonema Hua contains cofactor binding motif and catalytic tetrad motif,and its protein expression products have stable physical and chemical properties.
作者
程鹤
祝明珠
徐君
刘峻麟
俞年军
彭代银
周安
吴振东
韩荣春
CHENG He;ZHU Ming-zhu;XU Jun;LIU Jun-lin;YU Nian-jun;PENG Dai-yin;ZHOU An;WU Zhen-dong;HAN Rong-chun(School of Pharmacy, Anhui University of Chinese Medicine, Anhui Hefei 230012, China;Institute of Traditional Chinese Medicine Resources Protection and Development, Anhui Academy of Chinese Medicine, Anhui Hefei 230012, China;Qingyang Jiuhua Chinese Herbal Medicine Technology Co., Ltd., Anhui Chizhou 242800, China)
出处
《安徽中医药大学学报》
CAS
2021年第2期77-82,共6页
Journal of Anhui University of Chinese Medicine
基金
国家重点研发计划项目(2017YFC1701600,2017YFC1701602)
2017年中医药公共卫生服务补助专项(财社〔2017〕66号)
安徽省重大科技专项项目(202003a07020011)。
