摘要
目的观察肝细胞癌中组蛋白去甲基化酶含十字形结构域蛋白-3(JMJD3)和核因子-κB(NF-κB)蛋白水平的表达,探讨两者的相关性及其蛋白表达水平与多个临床病理因素的关系。方法采用实时荧光定量反转录-聚合酶链反应(RT-qPCR)法检测25例新鲜肝细胞癌组织及相对应的癌旁组织中JMJD3和NF-κB信使RNA(mRNA)表达,采用免疫组织化学法检测102例肝细胞癌及相对应的癌旁组织中JMJD3和NF-κB蛋白的表达,分析其与肝细胞癌临床病理特征的关系及上述蛋白的相关性,采用生存分析法(Kaplan-Meier)法来评估JMJD3和NF-κB蛋白的异常表达与肝细胞癌患者生存期之间的关系,Cox回归模型分析JMJD3和NF-κB蛋白的表达和临床病理参数与肝细胞癌预后的关系。结果JMJD3和NF-κB mRNA在肝细胞癌组织中的表达水平均高于癌旁组织(t=-15.130、-15.030,P<0.05),差异有统计学意义,肝细胞癌组织中JMJD3阳性率[87.25%(89/102)]显著高于相应癌旁组织[57.84%(59/102),χ^(2)=22.153,P<0.05],差异有统计学意义,肝细胞癌组织中NF-κB阳性率[83.33%(85/102)]显著高于相应癌旁组织[29.41%(30/102),χ^(2)=55.664,P<0.05],差异有统计学意义。JMJD3和NF-κB表达与甲胎蛋白水平、分化程度、有无脉管癌栓、淋巴结转移及TNM分期明显相关(P<0.05),NF-κB与结节数目明显相关(χ^(2)=5.133,P<0.05),差异有统计学意义。JMJD3蛋白高表达者的生存期高于低表达者(χ^(2)=40.568,P<0.05),差异有统计学意义,NF-κB蛋白高表达者的生存期低于低表达者(χ^(2)=48.803,P<0.05),差异有统计学意义。Spearman相关分析结果显示JMJD3与NF-κB呈正相关(r=0.627,P<0.05)。结论JMJD3和NF-κB在肝细胞癌中的异常表达与肿瘤的浸润、转移及无病生存期明显相关。
Objective To observe the expression of jumonji domain-containing3(JMJD3)and nuclear factor-κB(NF-κB)mRNA and protein in hepatocellular carcinoma(HCC),and to explore the correlation between the expression of JMJD3 and NF-κB protein and clinicopathological factors.Methods JMJD3 and NF-κB mRNA and protein levels in 25 fresh hepatocellular carcinoma and its adjacent tissues were detected by quantitative real-time reverse transcription-polymerase chain reaction(RT-qPCR)and immunohistochemistry respectively.The relationship between the expression of JMJD3 and NF-κB proteins and the clinicopathological features of HCC was analyzed.The Kaplan-Meier method was used to evaluate the relationship between the abnormal expression of JMJD3 andNF-κB proteins and the survival time of HCC patients.The Cox-regression model was used to evaluate the prognositic factors,including the expression of JMJD3 and NF-κB proteins and clinicopathological parameters.Results The expression levels of JMJD3 and NF-κB mRNA in HCC tissues were higher than those in adjacent tissues(P<0.05).The positive rate of JMJD3 in HCC tissues[87.25%(89/102)]was significantly higher than that in corresponding adjacent tissues[57.84%(59/102),χ^(2)=22.153,P<0.05].The positive rate of NF-κB in HCC tissues[83.33%(85/102)]was significantly higher than that in corresponding adjacent tissues[29.41%(30/102),χ^(2)=55.664,P<0.05].The expression of JMJD3 and NF-κB was correlated with AFP level,differentiation degree,vascular tumor thrombus,lymph node metastasis and TNM stage(P<0.05),and NF-κB was correlated with lymph node metastasis(χ^(2)=5.133,P<0.05).The survival time of patients with high expression of JMJD3 protein was shorter than that of patients with low expression(χ^(2)=40.568,P<0.05),and that of patients with high expression of NF-κB protein was shorter than that of patients with low expression(χ^(2)=48.803,P<0.05).Spearman correlation analysis showed that JMJD3 was positively correlated with NF-κB(r=0.627,P<0.05).Conclusion The abnormal expression of JMJD3 and NF-κB in HCC is closely related to tumor invasion,metastasis and disease free survival,which can be used as important indicators to judge the poor prognosis of HCC.
作者
王冰
李锡清
杨建旭
牛标
王维伟
孙建军
魏思东
陈国勇
Wang Bing;Li Xiqing;Yang Jianxu;Niu Biao;Wang Weiwei;Sun Jianjun;Wei Sidong;Chen Guoyong(Department of Hepatobiliary Pancreatic Surgery,Henan Provincical People′s Hospital,Zhengzhou 450003,China)
出处
《中华实验外科杂志》
CAS
北大核心
2021年第3期542-546,共5页
Chinese Journal of Experimental Surgery
基金
国家自然科学基金(U2004124)。
关键词
肝细胞癌
核因子-ΚB
Hepatocellular carcinoma
Nuclear factor-κB
