摘要
建立了超高效液相色谱-串联质谱(UPLC-MS/MS)测定甘蔗中11种多酚类物质含量的方法。样品经甲醇超声提取,采用UPLC-MS/MS进行分析检测,电喷雾负离子电离(ESI-),多反应监测(MRM)检测,外标法定量。该方法在5~200μg/L范围内线性良好(r^(2)>0.999),检出限为0.5~17.0μg/kg。在样品中添加100μg/kg的11种多酚标准溶液,回收率为89.4%~102.4%,相对标准偏差为0.9%~4.3%(n=6)。此方法简便快捷,灵敏度高,适用于甘蔗中多酚类物质的定性定量分析。
A method was developed for the determination of 11 polyphenolic compounds in sugarcane by ultra performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS).The samples were extracted by ultrasonic methanol,analyzed and detected by UPLC-MS/MS,electrospray negative ion ionization(ESI-),multi-reaction monitoring(MRM)and detection,and quantified by external standard method.The method showed good linearity in the range of 5~200μg/L(r^(2)>0.999),and the detection limit was 0.5~17.0μg/kg.The recoveries of 11 polyphenols were 89.4%~102.4%with the relative standard deviations of 0.9%~4.3%(n=6)when 100μg/kg of 11 standard solutions were added to the samples.This method was simple,quick and sensitive,and suitable for the qualitative and quantitative analysis of polyphenols in sugarcane.
作者
王小鹏
黄敏兴
许卓妍
杨李胜
刘志鹏
余构彬
WANG Xiao-peng;HUANG Min-xing;XU Zhuo-yan;YANG Li-sheng;LIU Zhi-peng;YU Gou-bin(Institute of Bioengineering,Guangdong Academy of Sciences,Guangzhou 510316;Research Center for Sugarcane Industry Engineering Technology of Light Industry of China,Guangzhou 510316)
出处
《甘蔗糖业》
2021年第2期72-77,共6页
Sugarcane and Canesugar
基金
广州市科技计划项目(201806010093)。
