摘要
探讨柴桂口服液在脂多糖(LPS)诱导RAW 264.7细胞中的抗炎能力,为研发具有抗炎功效的柴桂制剂提供依据和参考。利用LPS刺激RAW 264.7细胞建立体外炎症模型,CCK8法检测药物对细胞的安全浓度,观察不同浓度的柴桂口服液作用细胞后的体外抗炎效果。结果表明,柴桂口服液对RAW 264.7细胞安全浓度为不超过100μg/mL;在安全浓度范围内,柴桂口服液能抑制由LPS引起的细胞促炎因子IL-6、IL-8、IL-1β、TNF-α及NO生成,降低IL-6、IL-8、IL-1β、TNF-α及iNOS表达,从而调节炎性因子平衡,抑制炎症反应。研究结果表明,柴桂口服液能有效减缓由脂多糖诱导引起的细胞炎症反应。
To investigate the anti-inflammatory effect of Chaigui oral liquid in lipopolysaccharide(LPS)induced RAW264.7 cells,and to provide basis and reference for the development of Chaigui preparation,in this study,LPS was used to stimulate RAW264.7 cells to establish an in vitro inflammatory model.The safe concentration of drug on cells was detected by CCK8 method,and the anti-inflammatory effect of different concentrations of Chaigui oral liquid on the cells was observed.The safe concentration of RAW 264.7 cells was less than 100μg/mL.Within the safe concentration range,Chaigui oral liquid can inhibit the production of pro-inflammatory cytokines such as IL-6,IL-8,IL-1β,TNF-αand NO induced by LPS,reduce the expressions of IL-6,IL-8,IL-1β,TNF-αand iNOS,so as to regulate the balance of inflammatory factors and inhibit inflammatory response.In brief,Chaigui oral liquid can effectively decrease the cell inflammatory response induced by lipopolysaccharide.
作者
周淑棉
刘梦倩
樊丹阳
胡庭俊
ZHOU Shu-mian;LIU meng-qian;FAN Dan-yang;HU Ting-jun(College of Animal Science and Technology,Guangxi University,Nanning,Guangxi 530005,China;Library of Guangxi University,Nanning,Guangxi 530004,China)
出处
《动物医学进展》
北大核心
2021年第5期80-84,共5页
Progress In Veterinary Medicine
基金
广西创新驱动发展专项资金项目(桂科AA17204081-2)
国家现代农业产业技术体系广西创新团队建设专项(nycytxgxcxtd-14-02)。
