摘要
为筛选适合龙牙楤木中酚类物质的提取方法。本文以龙牙楤木嫩叶为原料,采用溶剂浸提法、溶剂回流法、超声辅助法、微波辅助法以及复合酶法提取龙牙楤木嫩叶中酚类物质,对酚类物质的得率进行比较分析,筛选出最佳提取方法,并在其单因素基础上,进一步利用正交试验优化各工艺参数。结果表明,五种提取方法中复合酶法提取酚类物质效果最好;正交试验得到复合酶法提取的最佳工艺参数为:复合酶(果胶酶和各种碳水化合物酶组成)用量120μL/g(酶量/样品量),酶解温度50℃,酶解pH6,酶解时间1.5 h,乙醇体积分数50%,此条件下龙牙楤木中总酚得率为7.24%,总黄酮得率为3.17%。通过正交实验优化复合酶法提取龙牙楤木嫩叶中酚类物质,在最佳工艺参数下提取的效果理想,可用于后续对酚类物质纯化、鉴定等研究。
For the purpose of promoting the extraction rate of phenolic compounds from Aralia elata(Miq.)Seem.,five methods including solvent extraction,solvent reflux extraction,ultrasonic-assisted extraction,microwave-assisted extraction and combined-enzyme extraction were respectively applied.After comparing the extraction rate of the five methods,then the most suitable method was obtained and orthogonal experiment was carried out to determine its optimum parameters.Result showed that the combined-enzyme extraction is more suitable for the extraction of phenolic compounds compared with other methods.According to the orthogonal experiment,its optimum extraction parameters were as follows:Enzyme dosage(composed of pectinase and various carbohydrate enzymes)120μL/g(enzyme amount/sample weight),enzymatic hydrolysis temperature 50℃,pH6,enzymatic hydrolysis time 1.5 hours,ethanol concentration of 50%.Finally,under the aforementioned optimum technology conditions,the extraction rate of total phenols from the powder of Aralia elata(Mip.)seem.was 7.24%and total flavonoids was 3.17%.In conclusion,through the orthogonal experiment and combined-enzyme extraction,phenolic compounds from Aralia elata obtained the ideal extraction effect which could be studied for further purification and identification experiment.
作者
朱定波
徐卫
ZHU Dingbo;XU Wei(The Ninth People’s Hospital of Chongqing,Chongqing 400700,China)
出处
《食品工业科技》
CAS
北大核心
2021年第10期189-194,共6页
Science and Technology of Food Industry
关键词
龙牙楤木
总酚
总黄酮
复合酶法
Aralia elata(Miq.)Seem.
total phenols
total flavonoids
combined-enzyme extraction
