摘要
目的获得南方汉族群体的基因多态性信息,分析16个东亚各人群的族源关系。方法 2018年3~7月收集贵州省和江西省汉族群体中健康且无亲缘关系的720份个体血液样本,其中贵州省407份,江西省313份。使用短串联重复序列(STR)试剂盒扩增检测样本,获得法医学参数;通过文献获取湖北汉族,湖南汉族,四川汉族,重庆汉族,贵州布依族、侗族和苗族,云南白族、彝族、哈尼族和纳西族,广西壮族以及日本和韩国共14个人群的法医学参数,采用Arlequin v3.5遗传软件计算16个东亚人群(本研究的贵州汉族、江西汉族以及文献获得的14个人群)之间的相对遗传距离(Fst),采用SPSS 21.0统计学软件进行多维尺度分析(MDS)和主成分分析(PCA),MEGA6软件绘制系统进化树。结果在贵州汉族人群中,累积个人识别率为1-3.3080×10-23,累积非父排除率为1-3.1792×10-8。在江西汉族人群中,累积个人识别率为1-5.4721×10-23,累积非父排除率为1-1.6544×10-8。少数民族(贵州布依族、贵州侗族、贵州苗族、广西壮族、云南哈尼族和云南纳西族)与汉族群体间存在明显的遗传距离。日本人群与汉族群体的遗传距离最大,韩国人群与汉族群体具有较近的遗传距离。进化树结果表明贵州汉族、江西汉族群体与其他汉族群体聚集在一起,未见明显区别。结论 14个基因座在贵州、广西人群中遗传多态性较好,少数民族与汉族之间、日本与汉族之间的遗传差异明显,而汉族人群内部遗传差异不明显。
Objective To obtain the genetic polymorphism information Han population in South China and analyse phylogenetic structure among some eastasian populations. Methods 407 blood samples of unrelated healthy individuals from Guizhou Han population and 313 blood samples of unrelated healthy individuals from Jiangxi Han population were collected after obtaining informed consent. The forensic parameters of 14 populations, including Hubei Han, Hunan Han, Sichuan Han, Chongqing Han, Guizhou Buyi, Dong and Miao, Yunnan Bai, Yi, Hani and Naxi, Guangxi Zhuang, Japan and South Korea, were obtained through literature. Forensic genetic characteristics of the two studied populations and phylogenetic relationship among the 16 populations were analysed. Results For Guizhou Han population, the combined power of discrimination(CPD)was 1-3.3080×10-23 and the combined power of exclusion(CPE) was 1-3.1792×10-8. For Jiangxi Han populations, CPD was 1-5.4721×10-23 and CPE was 1-1.6544×10-8. Then, using pairwise genetic distances, phylogenetic analysis, multidimensional scaling(MDS) and principal component analysis(PCA), significant genetic differences existed between minority ethnic groups(particularly in Guizhou Bouyei, Guizhou Dong, Guizhou Miao, Guangxi Zhuang, Yunnan Hani, and Yunnan Nakhi) and Han populations. Japanese were far from Chinese population. South Korean were close to Chinese Han population. The studied Guizhou Han and Jiangxi Han populations were not differentiated fromother Han populations. Conclusions In Guizhou and Guangxi populations, 14 genetic loci are good for individual discrimination and non-parent exclusion. The difference between minorities and Han population, between Japanese and Han populations is significant. The six Han populations can’t be differentiated from each other. In order to provide more information for population genetics studies, more in-depth statistical analysis of our genetic data and larger sample size of some ethnicities is required.
作者
贾振军
高春芳
钱尊磊
刘卓
金华
苑美青
Jia Zhenjun;Gao Chunfang;Qian Zunlei;Liu Zhuo;Jin Hua;Yuan Meiqing(Institute of Criminal Investigation Science College,People's Public Security University of China,Beijing 100038,China;School of Information Technology and Cyber Security,People's Public Security University of China;Institute of Forensic Science,Key Laboratory of Forensic Genetics,Ministry of Public Security,Beijing 100038,China)
出处
《中国法医学杂志》
CSCD
2021年第2期162-166,172,共6页
Chinese Journal of Forensic Medicine
基金
中国人民公安大学基本科研业务费项目(2019JKF217)
中国人民公安大学校级教改研究课题(2019JY33)
国家安全高精尖学科高端论文资助项目(2020GDLW030)
国家重点研发计划项目(2018YFC0824405-04)。
关键词
基因
法医学
短串联重复序列
Gene
Forensic science
Short tandem repeat
