摘要
为解决宁夏地区番茄种质资源数量多、保存难度高、更新负担大、优良品种选育效率低等问题,本研究通过对480份番茄种质资源的20个表型性状进行田间调查,采用相关性分析、主成分分析、聚类分析进行遗传多样性分析;利用欧氏距离和不加权类平均法,分别选用随机取样法、优先取样法和偏离度取样法,按10%取样比例分别构建核心种质,并对其进行代表性评价。结果表明:该480份番茄种质资源具有丰富的遗传多样性,在欧式距离为1.25时,分为6大类群;成功构建出3组核心种质R1、P1和D1,其中,分别通过随机取样法和偏离度取样法构建的R1和D1符合核心种质的构建原则;3组核心种质均具有良好的异质性,且通过优先取样法构建的P1获得了更大的遗传变异。本研究构建的宁夏地区番茄核心种质,可为该地区番茄种质资源的保存和新品种选育提供科学方法,也可为该种质资源的相关研究提供理论参考。
In order to solve the problems of large quantity of tomato germplasm resources,high conservation difficulty,heavy renewal burden and low breeding efficiency for fine varieties in Ningxia region,the genetic diversity of 20 phenotypic traits for 480 tomato germplasm resources was analyzed by correlation analysis,principal component analysis and cluster analysis.Euclidean distance and unweighted pair-group method with arithmetic means(UPGMA)were used,and then the methods of random sampling,preferential sampling and deviation sampling were separately used to construct core collections according to 10%sampling ratio,and the representation of core collections was evaluated.The results showed that the 480 tomato germplasms had rich genetic diversity and could be divided into six groups when the Euclidean distance was 1.25.Three groups of core collections R1,P1 and D1 were successfully constructed,among which R1 and D1 separately constructed by random sampling method and deviation sampling method were consistent with the construction principle of core collection,and all the three groups of core collections had good heterogeneity,and P1 constructed by preferential sampling method obtained greater genetic variation.In a word,the core collections of tomato germplasm resources constructed in Ningxia provide scientific methods for the conservation of tomato germplasm resources and breeding of new varieties,and also provide theoretical references for the related research of germplasm resources.
作者
郑福顺
王晓敏
李国花
李洪磊
周鹏泽
王林
白圣懿
刘珮君
张雪艳
胡新华
付金军
高艳明
李建设
ZHENG Fushun;WANG Xiaomin;LI Guohua;LI Honglei;ZHOU Pengze;WANG Lin;BAI Shengyi;LIU Peijun;ZHANG Xueyan;HU Xinhua;FU Jinjun;GAO Yanming;LI Jianshe(School of Agriculture,Ningxia University,Yinchuan 750021,China;Ningxia Modern Facility Horticulture Engineering and Technology Research Center,Yinchuan 750021,China;Key Laboratory of Modern Molecular Breeding for Dominant and Special Crops in Ningxia,Yinchuan 750021,China;Ningxia Facility Horticulture Technology Innovation Center(Ningxia University),Yinchuan 750021,China;Ningxia Jufeng Seedlings Limited Liability Company,Yinchuan 750021,China)
出处
《浙江大学学报（农业与生命科学版）》
CAS
CSCD
北大核心
2021年第2期171-181,共11页
Journal of Zhejiang University:Agriculture and Life Sciences
基金
国家自然科学基金(31860561)
宁夏回族自治区农业特色优势产业育种专项(NXNYYZ20200104)
宁夏回族自治区重点研发计划(重大)重点项目(2019BBF02022)。
关键词
宁夏
番茄
种质资源
表型性状
核心种质
Ningxia
tomato
germplasm resources
phenotypic traits
core collections
