摘要
目的观察胰岛素样生长因子1(IGF 1)、骨髓间充质干细胞(BMSCs)与丝素蛋白羟基磷灰石复合材料构建的组织工程骨修复骨缺损的效果。方法用45只新西兰大白兔制备骨缺损模型后随机分为3组,每组15只,A组骨缺损处不植入任何材料,B组骨缺损处植入单纯骨髓间充质与丝素蛋白羟基磷灰石复合材料复合体,C组骨缺损处植入IGF 1转染的BMSCs与丝素蛋白羟基磷灰石复合材料复合体。造模后4、8、12周,分别进行X线片拍摄、苏木精-伊红染色及三点弯曲实验,组间比较采用t检验。结果造模后4周,3组均可见骨痂形成;造模后12周,A组未形成完整的骨桥,B组骨痂开始塑形,骨髓腔基本再通,C组完成骨缺损修复。B、C组最大载荷逐渐增加,C组最大载荷始终高于B组(t_(8)=5.208,t_(12)=12.837,P值均<0.05)。造模后4周,A组可见纤维组织增生与少量骨小梁形成;B、C组复合支架材料部分降解,可见骨小梁形成并相互连接。造模后12周,A仍为较多的纤维组织,B组开始塑形为皮质骨,C组基本形成新的皮质骨。结论IGF 1转染的BMSCs与丝素蛋白羟基磷灰石复合材料构建的组织工程骨,具有比单纯BMSCs与丝素蛋白羟基磷灰石复合材料构建的组织工程骨更强大的骨诱导能力。
Objective To observe the effect of tissue engineering bone repair of bone defect constructed by insulin-like growth factor 1(IGF 1),bone marrow mesenchymal stem cells(BMSCs)and fibroin hydroxyapatite composite material.Methods A total of 45 New Zealand white rabbits after the preparation of bone defect model were randomly divided into three groups,each group of 15:group A,bone defect without no implantation of material;group B,bone defect with implantation of BMSCs and silk fibroin hydroxyapatite composite complex;group C,bone defect with implantation of BMSCs transfected with IGF 1 and silk fibroin hydroxyapatite composite complex.At 4,8 and 12 weeks after modeling,X-ray film,hematoxylin-eosin staining and three-point bending experiments were performed.SPSS 21.0 software was used for statistical analysis.Results Four weeks after modeling,callus formation was observed in all three groups.After 12 weeks of modeling,no complete bone bridge formed in group A,callus began to shape in group B,bone marrow cavity was basically recanalized,and bone defect repair was completed in group C.The maximum load in group B and group C gradually increased,and the maximum load in group C was significantly higher than that in group B(t_(8)=5.208,t_(12)=12.837,P<0.05).Four weeks after modeling,fibrous tissue hyperplasia and a small amount of bone trabecular formation were observed in group A.The composite scaffold materials in group B and group C were partially degraded,and bone trabeculae formed and were connected each other.After 12 weeks of modeling,there was abundant fibrous tissue in group A,cortical bone was observed in group B,and new cortical bone basically formed in group C.Conclusion The tissue-engineered bone constructed by IGF-1-transfected BMSCs and fibroin hydroxyapatite composite material has stronger bone induction capability than the tissue-engineered bone constructed by simple BMSCS and fibroin hydroxyapatite composite material.
作者
李明凡
冯大雄
周庆忠
郑礼鹏
汪在德
Li Mingfan;Feng Daxiong;Zhou Qingzhong;Zheng Lipeng;Wang Zaide(Department of Spinal Surgery,Affiliated Hospital of Southwest Medical University,Luzhou 646000,China)
出处
《中华实验外科杂志》
CAS
北大核心
2021年第4期712-714,共3页
Chinese Journal of Experimental Surgery
