摘要
目的通过采集大气中的总悬浮颗粒(TSP),经液相萃取和高效色谱-荧光法,建立一种大气中环境内分泌干扰物的检测方法。方法大气以中流量TSP采样器100 L/min的流速通过玻璃纤维滤纸采样2 h,滤纸样品经提取剂二氯甲烷-丙酮(3∶2,v/v),超声30 min后2500 r/min离心20 min,取上清液,55℃水浴蒸干,加乙腈定容。色谱条件:荧光检测法(λex=275 nm,λem=312 nm)。结果标准系列中BPA、OP和NP含量与各自对应峰面积呈良好的线性关系,相关系数均>0.999,检出限为9.8 ng/mL、5.6 ng/mL、5.3 ng/mL,回收率范围分别为92.1%~127.0%、83.9%~97.4%、83.7%~101.2%,日内精密度为0.67%、1.10%、2.33%,日间精密度为2.09%、4.36%、2.21%,均<5%。结论本方法简便、快速、准确,可用于大气环境中BPA、OP和NP的检测。
Objective To establish a convenient and efficient method to detect Environmental endocrine disruptors(EDCs)in atmospheric particulate matter.Methods Atmospheric total suspended particles(TSP)was sampled through glass fiber filter paper in a medium-flow TSP sampler at a flow rate of 100 L/min for 2 hours.The filter paper sample was then subjected to the treatments as follows:(1)The samples were extracted with acetone and dichloromethane(3∶2,v/v)from glass fiber filter paper.(2)Ultrasonic cleaning for 30 minutes.(3)Centrifugation at 2500 r/min for 20 minutes.(4)The supernatant was dried out in water baths at 55℃,and then acetonitrile was added to make the volume constant.Chromatographic condition was:HPLC-FLD(λex=275nm,λem=312nm).Results The content of OP,NP and BPA in the standard series showed a good linear relationship with their respective peak areas,and all the correlation coefficients were greater than 0.99.The detection limits for BPA,OP,NP were 9.80 ng/mL,5.60 ng/mL,and 5.30 ng/mL,respectively,and the recoveries were 92.10%~127.00%,83.90%~97.40%,and 83.70%~101.20%,respectively.The RSD for the inter-and intra-day was less than 5%.Conclusion The method was simple,rapid,and accurate,which could be used for the detection of environmental endocrine disruptors in atmospheric particulate matter.
作者
谢松颖
杨昱
何洁
韦翼丹
俞捷
许洁
罗娅
XIE Songying;YANG Yu;HE Jie;WEI Yidan;YU Jie;XYU Jie;LUO Ya(School of Public Health,Zunyi Medical University,Zunyi,Guizhou 563099,China)
出处
《公共卫生与预防医学》
2021年第3期33-36,共4页
Journal of Public Health and Preventive Medicine
基金
遵义医科大学创新创业训练计划项目(ZYDC2018019)
贵州省大学生创新创业训练计划项目(20195200992)
遵义医科大学硕士启动基金(F-836)。
