摘要
目的探讨miR-144-3p对前列腺癌PC3细胞增殖和侵袭的影响及可能机制。方法利用脂质体介导的方法进行转染,将前列腺癌PC3细胞分为空白组(未进行转染)、miR-144-3p阴性对照组(转染无义序列)和miR-144-3p模拟物组(转染miR-144-3p模拟物),采用实时定量PCR验证转染效率,Western blot检测各组PC3细胞RUNX1蛋白表达,CCK-8法检测各组PC3细胞增殖能力变化,Transwell检测各组细胞侵袭能力变化,应用生物信息学网站分析RUNX1是否为miR-144-3p潜在的靶基因,应用双荧光素酶报告基因实验验证。结果 miR-144-3p过表达能抑制PC3细胞体外增殖和侵袭能力,PC3细胞中RUNX1蛋白表达水平下降;生物信息学分析结果显示RUNX1基因3’UTR区存在miR-144-3p的结合位点,双荧光素酶实验证实RUNX1为miR-144-3p的靶基因。结论 miR-144-3p通过靶向RUNX1抑制前列腺癌PC3细胞的增殖与侵袭。
Objective To investigate the effect and mechanism of miR-144-3 p on the proliferation and invasion of prostate cancer PC3 cells. Methods PC3 cells were transfected with liposome-mediated, and the PC3 cells were divided into blank control group(not transfected), miR-144-3 p negative control group(transfected unrelated sequence), miR-144-3 p mimics group(transfected miR-144-3 p mimics). Real time PCR was used to verify the transfection efficiency. The proliferation and invasion of PC3 cells were detected by CCK-8 and transwell assay respectively, and the expression of RUNX1 protein was detected by Western blot. Bioinformatics and dual luciferase reporter gene experiment was used to verify whether RUNX1 is a target gene of miR-144-3 p. Results Overexpression of miR-144-3 p inhibited the proliferation and invasion of PC3 cells, decreased the expression level of RUNX1 protein. Bioinformatics and dual luciferase reporter gene confirmed that RUNX1 was the target gene of miR-144-3 p. Conclusion miR-144-3 p could inhibit the proliferation and invasion of prostate cancer PC3 cells via targeting RUNX1.
作者
赵兴亮
张帆
史晓宇
刘佳
ZHAO Xing-liang;ZHANG Fan;SHI Xiao-yu;LIU Jia(Department of Urology,Benxi Central Hospital,Benxi 117000;Liaoning Technology Innovation and R&D Engineering Center,Shenyang 110168;Laboratory Animal Center,China Medical University,Shenyang 110122,China)
出处
《解剖科学进展》
CAS
2021年第2期222-225,共4页
Progress of Anatomical Sciences
基金
辽宁省自然科学基金(2015020518)。