摘要
Tooth root morphogenesis involves two biological processes,root elongation and dentinogenesis,which are guaranteed by downgrowth of Hertwig’s epithelial root sheath(HERS)and normal odontoblast differentiation.Ubiquitin-dependent protein degradation has been reported to precisely regulate various physiological processes,while its role in tooth development is still elusive.Here we show ubiquitinspecific protease 34(USP34)plays a pivotal role in root formation.Deletion of Usp34 in dental mesenchymal cells leads to short root anomaly,characterized by truncated roots and thin root dentin.The USP34-deficient dental pulp cells(DPCs)exhibit decreased odontogenic differentiation with downregulation of nuclear factor I/C(NFIC).Overexpression of NFIC partially restores the impaired odontogenic potential of DPCs.These findings indicate that USP34-dependent deubiquitination is critical for root morphogenesis by stabilizing NFIC.
基金
supported by grants from the National Natural Science Foundation of China,(NSFC 81722014,81970913,81700980,and 81901042)
West China Hospital of Stomatology(RD-03-202010,RCDWJS2020-23)
the China Postdoctoral Science Foundation Grant(No.2019TQ0218)
State Key Laboratory of Oral Diseases(SKLOD202008).
