摘要
采用超声提取结合高效液相色谱同时测定纺织品中5种同分异构CMR物质[苯并(j)荧蒽(BjF)、苯并(e)芘(BeP)、苯并(b)荧蒽(BbF)、苯并(k)荧蒽(BkF)和苯并(a)芘(BaP)]的含量。样品0.500 g经20 mL体积比为1:1的甲醇-四氢呋喃混合溶剂于30℃超声提取30 min,提取液在Agilent Eclipse PAH色谱柱上分离,以乙腈-水混合液为流动相进行梯度洗脱,采用荧光检测器进行检测。结果表明:5种同分异构CMR物质的质量浓度在5.0~150μg·L^(-1)内与其峰面积呈线性关系,检出限(3S/N)均为1.0μg·L^(-1)。加标回收率为98.6%~103%,测定值的相对标准偏差(n=6)为2.9~3.7%。
HPLC combined with ultrasonic extraction was applied to the simultaneous determination of five isomeric CMRs(including BjF,BeP,BbF,BkF,and BaP)in textiles.The sample(0.500 g)was extracted by ultrasonic with 20 mL of the mixed solvent of methanol and tetrahydrofuran with volume ratio of 1:1 for 30 min at30℃.The extract was separated on an Agilent Eclipse PAH chromatographic column with the mixture of acetonitrile and water as the mobile phase in gradient elution,and fluorescence detector was used for detection.As shown by the results,linearity relationships between values of peak area and mass concentration of five isomeric CMRs were found in the same range of 5.0-150μg·L^(-1),and detection limits(3 S/N)were all 1.0μg·L^(-1).Recoveries obtained by standard addition method were in the range of 98.6%-103%and RSDs(n=6)of measured values were in the range of 2.9%-3.7%.
作者
秦鑫
徐韵扬
吴怡
顾虎
QIN Xin;XU Yunyang;WU Yi;GU Hu(Hangzhou Institute of Test and Calibration for Quality and Technology Supervision,Hangzhou 310019,China)
出处
《理化检验（化学分册）》
CAS
CSCD
北大核心
2021年第4期350-353,共4页
Physical Testing and Chemical Analysis(Part B:Chemical Analysis)
基金
国家重点研发项目(2017YFF0209600)
浙江省质量技术监督系统科研项目(20190119)
中国标准化研究院国家标准馆项目(JXJY-2017-266)。
