摘要
利用EST-SSR分子标记对以福鼎大白茶和安徽1号为母本,保靖黄金茶1号为父本的2个杂交F1进行真假杂种的鉴定和遗传多样性分析,结果表明:以福鼎大白茶为母本的48株F1中共鉴定出真杂种41株,杂种率为85.42%,以安徽1号为母本的44个F1中共鉴定出真杂种35株,杂种率79.55%。筛选出的17对SSR引物在2个杂交群体中共享等位基因41个,平均含有2.82(福鼎大白茶)和2.71(安徽1号)个,具有相对较高的多态性。2个杂交F1群体的遗传多样性参数差别不大,Nei、He、Ho、I平均值分别为0.56、0.43、0.78、0.93(福鼎大白茶×保靖黄金茶1号)和0.55、0.43、0.75、0.90(安徽1号×保靖黄金茶1号)。福鼎大白茶×保靖黄金茶1号和安徽1号×保靖黄金茶1号杂交F1中遗传相似系数的变化幅度分别在0.44~0.90和0.48~0.95之间,具有较丰富的遗传变异。根据相似系数矩阵按UPGMA进行聚类,2个杂交群体分别在相似系数为0.60和0.64处分为3大类:2个杂交子代各先聚为一类,然后再与母本聚为一类,最后与父本聚为一类,说明这2个杂交F1具有偏母本遗传倾向。该研究表明EST-SSR可应用于茶树真假杂种的鉴定。
EST-SSR molecular markers were used to identify the hybrids of two F1 crosses,which was derived from the variety Fuding Dabaicha and Anhui 1 as the female parent and Baojing Huangjincha 1 as the male parent,followed by a genetic diversity. The results showed that 41(85.42%)of 48 progenies from Fuding Dabaicha were identified to be true F1 hybrids,while among 35 strains(79.55%)of 44 plants from Anhui 1 were true hybrids. Genetic analysis using 17 pairs of SSR primers revealed 41 alleles in the two hybrid populations with an average of 2.82(Fuding Dabaicha)and 2.71(Anhui 1). No significant difference on the genetic diversity parameters of the two hybrid F1 populations were detected. The mean values of Nei,He,Ho,I were 0.56,0.43,0.78,0.93(Fuding Dabaicha)and 0.55,0.43,0.75,0.90(Anhui 1),respectively. The variation range of genetic similarity coefficient in F1 hybrid of Fuding Dabaicha×Baojing Huangjincha 1 and Anhui 1×Baojing Huangjincha 1 was between 0.44-0.90 and 0.48-0.95 respectively. The cluster analysis using UPGMA showed that two hybrid populations were divided into three groups when the similarity coefficient was 0.60 and 0.64 respectively. The progenies were clustered together and represented a higher genetic similarity with respective female parents than the male parent. Collectively,this study reported an example on the genetic classification of tea hybrids using EST-SSR markers.
作者
雷雨
段继华
黄飞毅
康彦凯
罗意
陈宇宏
丁玎
姚利娜
董丽娟
李赛君
LEI Yu;DUAN Ji-hua;HUANG Fei-yi;KANG Yan-kai;LUO Yi;CHEN Yu-hong;DING Ding;YAO Li-na;DONG Li-juan;LI Sai-jun(Tea Research Institute,Hunan Academy of Agriculture Sciences/National Center for Tea Improvement,Hunan Branch/Hunan Observation and Experiment Station of Tea Plant and Tea Processing,Ministry of Agriculture/Hunan Engineering Technology Research Center for Tea Variety and Seedling,Changsha 410125)
出处
《植物遗传资源学报》
CAS
CSCD
北大核心
2021年第3期748-757,共10页
Journal of Plant Genetic Resources
基金
湖南省自然科学基金(2018JJ3268)
中央引导地方资金(2019XF5041)
湖南省农业科技创新项目(2020CX035)。
