摘要
目的探讨临床间接免疫荧光法和临床酶联免疫吸附试验对抗中性粒细胞胞质抗体临床检测方法的结果比较。方法选取2018年1月至2019年1月在沈阳金域医学检验所有限公司实验室诊断部的抗中性粒细胞胞质抗体临床检测样本200例,并进行临床间接免疫荧光法(IIF)和临床酶联免疫吸附法(ELISA)检测抗中性粒细胞胞质抗体(ANCA)的临床一致性比较。结果两种临床方法的一致性较好且差异具有临床统计学意义(P<0.05)。结论临床间接免疫荧光法和临床酶联免疫吸附试验法单独检测抗中性粒细胞胞质抗体均有漏检,临床联合检测的效果最佳,值得在临床上进一步推广。
Objective To compare the clinical results of indirect immunofluorescence and enzyme-linked immunosorbent assay(elisa)against neutrophils.Methods From January 2018 to January 2019 in Shenyang Golden Field Medical Institute CO.,Ltd.Department of laboratory diagnosis of neutrophils resistant cytoplasm antibodies,200 cases of clinical test sample and clinical indirect immunofluorescence(IIF)and clinical enzyme-linked immunosorbent(ELISA)to detect anti neutrophil cytoplasmic antibodies(ANCA)clinical consistency.Results The consistency of the two clinical methods was good and the difference was statistically significant(P<0.05).Conclusion Both indirect immunofluorescence and enzyme-linked immunosorbent assay have missed detection,and the combined clinical detection has the best effect and can be further promoted in clinical practice.
作者
于潇
YU Xiao(Laboratory Diagnostic Department,Shenyang Jinyu Medical Laboratory Co.,Ltd.,Shenyang 110164,China)
出处
《中国医药指南》
2021年第11期132-133,共2页
Guide of China Medicine
关键词
间接免疫荧光法
酶联免疫吸附试验
抗中性粒细胞胞质抗体检测
Indirect immunofluorescence
Enzyme-linked immunosorbent assay
Detection of anti-neutrophilic cytoplasmic antibody
