摘要
为探索天然酚类化合物百里香酚、和厚朴酚与化学合成杀菌剂百菌清、啶酰菌胺混配的增效作用,筛选出能够防治灰霉病的混剂,本研究将百里香酚、和厚朴酚分别与百菌清、啶酰菌胺以不同比例混配,采用菌丝生长速率法测定其对灰葡萄孢菌Botrytis cinerea的抑菌活性,并将Bliss法、Mansour法和孙云沛法结合,以优化筛选方案,评价混剂的联合毒力。实验结果表明:将Bliss法、Mansour法和孙云沛法结合使用能够有效降低筛选工作量,且准确率较高;百里香酚和百菌清以质量比5:1、2:3、1:5和1:10比例混配,和厚朴酚和百菌清以质量比10:1、5:1、3:2、2:3和1:10比例混配均表现出协同增效作用;和厚朴酚和百菌清以10:1比例混配时协同增效作用最明显,其CTC值为309.15,以1:10比例混配时抑菌活性最高,其对B.cinerea的EC50为0.29 mg/L,高于杀菌剂百菌清。因此,将定性分析方法与定量分析方法结合使用是筛选杀菌剂混剂配方的合理方案,天然酚类化合物百里香酚、和厚朴酚与百菌清以合理比例混配所得混剂具有防治灰霉病的潜力。
In order to explore the synergistic effect of the mixtures of natural phenolic compounds(thymol and honokiol)with synthetic fungicides(chlorothalonil and boscalid)and screen out the novel fungicide mixtures which could control gray mold,in this study,thymol and honokiol were mixed with chlorothalonil and boscalid in different proportions,respectively,and the antifungal activity of the mixtures against Botrytis cinerea was determined by mycelium growth rate method.The Bliss’s method,Mansour’s method and Sun’s method were combined to optimized screening program,and the combined toxicity of the fungicide mixtures was evaluated.The results showed that the combination of Bliss’s method,Mansour’s method and Sun’s method could effectively reduce the screening workload and achieve high accuracy.The mixtures of thymol and chlorothalonil in mass ratios of 5:1,2:3,1:5,1:10,and the mixtures of honokiol and chlorothalonil in mass ratios of 10:1,5:1,3:2,2:3 and 1:10 showed a good synergistic effect.Among the mixtures of honokiol and chlorothalonil,the mass ratio of 10:1 showed the highest synergistic effect,with CTC value of 309.15.The mixture of honokiol and chlorothalonil in the mass ratio of 1:10 showed the highest antifungal activity,with EC50 value of 0.29 mg/L against B.cinerea,which was higher than that of chlorothalonil.Therefore,the combined use of the qualitative analysis methods and quantitative analysis methods is a proper scheme to screen the fungicide mixtures.The mixtures of natural phenolic compounds(thymol and honokiol)and chlorothalonil at reasonable ratios has the potential to control gray mold.
作者
李佳俊
陶丽红
叶敏
王凯博
吴文伟
何成兴
范黎明
苏发武
Li Jiajun;Tao Lihong;Ye Min;Wang Kaibo;Wu Wenwei;He Chengxing;Fan Liming;Su Fawu(State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan,Yunnan Agricultural University,Kunming 650201;Institute of Agricultural Environment and Resource,Yunnan Academy of Agricultural Sciences,Kunming 650205)
出处
《中国农学通报》
2021年第14期150-157,共8页
Chinese Agricultural Science Bulletin
基金
国家自然科学基金项目“牛至挥发油抑制果实采后致病真菌的物质基础及作用机制”(32060631)
云南省科技计划项目“天然单萜酚类化合物对杀菌剂的增效作用机制研究”(201901U070024)
国家重点研发计划项目“长江流域冬小麦化肥农药协同替代与减施增效技术研究”(2018YFD0200503)
国家重点研发计划项目“西南山地河谷稻区特色农药化肥减施增效技术集成与示范”(2018YFD0200308)。
