摘要
为了提供水环境中抗生素降解产物的抑菌性和生物毒性的监测与评估方法,本文结合非均相Fenton技术降解四环素废水实例,对抑菌试验菌种的选取和培养、试验步骤以及分析方法进行了阐述;结合实例反应的中间产物气相色谱-质谱法的解析结果,采用ECOSAR预测模型对降解后废水的毒性进行评估。结果表明,以大肠杆菌为指示菌的打孔法测定不同反应时间出水的抑菌圈大小出现明显变化,说明该抑菌试验法是一种简单、有效的测试水体抑菌性试验方法,适用于环境领域中低精度的抑菌能力检验;同时利用ECOSAR软件可对抗生素中间产物的水生生物毒性计算出响应值。两种方法结合可分别评估抗生素降解产物的抑菌性和生物毒性,有利于抗生素去除技术的进一步发展。
In order to provide a monitoring and evaluation method for the bacteriostatic and biological toxicity of antibiotic degradation products in the aquatic environment,combined with the heterogeneous Fenton technology for degrading tetracycline wastewater,this article explains the selection and cultivation of bacteriostatic test bacteria,test procedures and analysis methods.Combined with the analytical results of the intermediate product gas chromatography-mass spectrometry of the example reaction,the ECOSAR prediction model is used to evaluate the toxicity of the degraded wastewater.The results showed that the perforation method using E.coli bacteria as indicator bacteria to determine the size of the inhibition zone of the effluent at different reaction times showed significant changes,indicating that the bacteriostatic test method is a simple and effective test method for testing the bacteriostatic properties of water bodies,and it is applicable Low-precision antibacterial ability test in the environmental field;at the same time,the ECOSAR software can be used to calculate the response value of the toxicity of the aquatic organisms of antibiotic intermediates.The combination of the two methods can separately evaluate the bacteriostatic and biological toxicity of antibiotic degradation products,which is conducive to the further development of antibiotic removal technology.
出处
《福建建设科技》
2021年第3期96-98,共3页
Fujian Construction Science & Technology
