摘要
目的比较睾丸取精中应用新鲜精子和冷冻后复苏精子对胚胎发育及妊娠结局的影响。方法选取2015年1月1日至2018年7月31日在沈阳菁华医院生殖中心经睾丸切开取精(TESE)获精子行卵质内单精子注射(ICSI)助孕的782对夫妇的临床资料作为研究对象。根据精子来源不同分为两组,其中采用新鲜精子行ICSI纳入A组(n=637),采用冷冻复苏精子行ICSI纳入B组(n=145)。比较两组获卵率、卵子成熟率、受精率、异常受精率、卵裂率和优质胚胎率,及囊胚形成率和临床妊娠率。结果 B组卵裂率略高于A组,受精率和优质胚胎率略低于A组,但差异无统计学意义(P>0.05);B组囊胚形成率和临床妊娠率高于A组,差异具有统计学意义(P<0.05)。结论采用睾丸冷冻精子进行ICSI,其囊胚形成率和临床妊娠率高于新鲜精子。
Objective To analyze the effects of fresh sperm and cryopreservation sperm on embryo development and pregnancy outcome in testicular sperm extraction. Methods The clinical data of 782 couples who received intracytoplasmic sperm injection(ICSI) with testicular sperm extraction(TESE) in Shenyang Jinghua Hospital from January 1, 2015 to July 31, 2018 were selected and divided into two groups according to the source of sperm. Patients received fresh testicular sperm were included in group A(n=637), while patients received frozen testicular sperm were included in group B(n=145). The occytes retrieved rate, oocyte maturation rate, fertilization rate, abnormal fertillization rate, cleavage rate, high-quality embryo rate, clinical pregnancy rate and blastocyst formation rate were compared between the two groups. Results The cleavage rate in group B was slightly higher than that in group A, while the fertilization rate and high-quality embryo rate were slightly lower than that in group A, but the differences were not statistically significant(P>0.05). The blastocyst formation rate and clinical pregnancy rate in group B were significantly higher than that in group A(P<0.05). Conclusions The blastocyst formation rate and clinical pregnancy rate of ICSI using frozen testicular sperm were higher than fresh sperm.
作者
孟庆艳
程东凯
于洪君
李宝山
MENG Qingyan;CHENG Dongkai;YU Hongjun;LI Baoshan(Fertility Center,Shenyang Jinghua Hospital,Shenyang 110005,Liaoning,China)
出处
《中国性科学》
2021年第5期37-40,共4页
Chinese Journal of Human Sexuality
关键词
睾丸冻精
卵质内单精子注射
妊娠结局
Testicular frozen sperm
Intracytoplasmic sperm injection
Pregnancy outcome
