摘要
目的明确Th17/Treg免疫失衡与过敏性紫癜(Henoch-Schonlein purpura,HSP)合并肺炎支原体(Mpcoplasma pneumoniae,MP)感染之间的相关性。方法收集2018年6月至2019年12月期间于秦皇岛市第一医院与秦皇岛市妇幼保健院儿科诊断为HSP或MP感染的住院儿童和门诊进行健康体检的健康儿童为研究对象。根据是否诊断为HSP或MP感染将研究对象分为HSP组(41例)、MP组(25例)、HSP-MP组(19例)和正常对照组(20例)。应用流式细胞仪测定4组研究对象外周静脉血中Th17、Treg细胞百分率,ELISA法检测4组研究对象外周静脉血清中细胞因子白细胞介素(IL)-17、IL-6、转化生长因子(TGF)-β的浓度水平。结果4组患儿在年龄、性别分布上差异均无统计学意义(P>0.05)。比较外周血Th17细胞百分比以及IL-17、IL-6水平,HSP-MP组显著高于其余3组,差异均有统计学意义(P<0.05);HSP组、MP组两组均高于正常对照组,差异均有统计学意义(P<0.05)。比较外周血Treg细胞百分率,HSP-MP组显著低于其余3组,差异均有统计学意义(P<0.05);HSP组及MP组低于正常对照组儿童(P<0.05)。各组间TGF-β浓度差异无统计学意义(P>0.05)。结论高水平的IL-17与IL-6可能是引起HSP合并MP感染患儿严重的Th17/Treg免疫紊乱的重要因素,该免疫失衡可能在HSP合并MP感染发病中发挥重要作用。
Objective To discuss the correlation between the immune instability of Th17/Treg and the pathogenesis of the Henoch-Schonlein purpura(HSP)with Mpcoplasma pneumoniae(MP)infection.Methods From June 2018 to December 2019,children who had been diagnosed as HSP in the Department of Pediatrics at Qinhuangdao First Hospital and Qinhuangdao Maternal and Child Health Care Hospital,as well as in-patients with MP infection and outpatients,who had health check-up were collected.The subjects were divided into HSP group(41 cases),MP group(25 cases),HSP-MP group(19 cases)and control group(20 cases)according to the diagnosis of HSP and MP infection.The percentage of Th17 and Treg cells in peripheral venous blood of four groups were determined by flow cytometry,and the levels of interleukin(IL)-17,IL-6 and transforming growth factor-β(TGF-β)in peripheral venous serum of four groups were detected by ELISA.Results There were no statistically significant differences regarding age and gender distribution among four groups(P>0.05).The percentages of Th17 cells,IL-17 and IL-6 levels in peripheral blood of the HSP-MP group was significantly higher than those in the other three groups(P<0.05),which in HSP group and MP group were higher than that in control group(P<0.05).There was no statistical difference between HSP group and MP group(P>0.05).The percentage of Treg cells in peripheral blood was significantly lower in HSP-MP group than those in the other three groups(P<0.05),which in the HSP group and MP group were lower than those in control group(P<0.05).There was no statistical difference between HSP group and MP group(P>0.05).There were no statistically significant differences of TGF-βamong the four groups(P>0.05).Conclusion High levels of IL-17 and IL-6 may be an important factor causing severe Th17/Treg immune disorders in children with HSP combined with MP infection,which may play an important role in the pathogenesis of HSP combined with MP infection.
作者
钱宇
李秋平
高凤
王桂兰
Qian Yu;Li Qiuping;Gao Feng;Wang Guilan(Department of Pediatrics,the First Hospital of Qinhuangdao,Qinhuangdao 066001,China;Department of Clinical Laboratory,the First Hospital of Qinhuangdao,Qinhuangdao 066001,China;Department of Pediatrics,Qinhuangdao Maternal and Child Health Hospital,Qinhuangdao 066000,China)
出处
《中国小儿急救医学》
CAS
2021年第5期401-404,共4页
Chinese Pediatric Emergency Medicine
基金
秦皇岛市科学技术研究与发展计划(201805A145)。
关键词
儿童
过敏性紫癜
肺炎支原体
TH17
转化生长因子-Β
Children
Henoch-Schonlein purpura
Mycoplasma pneumoniae
Th17
Transforming growth factor-β
