摘要
目的:探索体外培养胎鼠真皮成纤维细胞(fibroblasts,FBs)皮内注射对小鼠背部光老化皮肤的改善作用。方法:分离培养10只第18.5天(E18.5)BALB/c胎鼠皮肤,体外培养FBs两天;用CM-Dil荧光染料进行活细胞标记,以示FBs注射后在体内存活情况。取BALB/c雄性小鼠20只,随机分为:未处理组5只、模型组5只(紫外线照射)、空白组5只(紫外线照射+注射PBS)、治疗组5只(紫外线照射+注射FBs),通过真表皮厚度及I型前胶原羧基端肽(PICP)的表达量评估胎鼠FBs对皮肤老化的改善作用。结果:胎鼠FBs形态多为梭型或不规则三角形,中央有卵圆形的细胞核,CM-Dil细胞示踪试验显示培养细胞皮内注射后第3天活性最强。治疗组小鼠真皮厚度(243.659±6.214)μm较空白组(126.198±12.352)μm明显增厚,差异有统计学意义(P<0.05)。治疗组PICP浓度(120.357±10.260)ng/mL高于空白组(95.357±13.471)ng/mL,差异有统计学意义(P<0.05)。结论:胎鼠FBs皮内注射可改善紫外线诱导的小鼠皮肤老化。
Objective:To investigate the effect of intradermal injection of fetal dermal fibroblasts(FBS)cultured in vitro on the photoaged dorsal skin of mice.Methods:The dermal fibroblasts were isolated and cultured from 10 BALB/c mouse embryo skins(E18.5)in vitro for two days.The living cells were labeled with CM-Dil dye to track the survival of FBs at the injection site.Twenty BALB/C male mice were randomly divided into 4 groups with 5 mice in each group:untreated group,model group(treated with ultraviolet),blank group(treated with ultraviolet+PBs injection)and treatment group(treated with ultraviolet+FBs).The effect of fetal FBs on skin photoaging was evaluated by the thickness of dermis and epidermis and the expression level of procollagen type IC-terminal propeptide(PICP).Results:FBS of fetal mice were mostly shuttle type or irregular triangle,with oval nucleus in the center.The number of CM-Dil-labeled cells was largest on the 3rd day post-injection.The dermis thickness of the treatment group was significantly thicker than that of the blank group(243.659±6.214μm vs.126.198±12.352μm)(P<0.05).The concentration of PICP in the treatment group(120.357±10.260)ng/mL was higher than that in the blank group(95.357±13.471)ng/mL with a significant difference(P<0.05).Conclusion:Intradermal injection of fetal FBS can improve the skin photoaging induced by ultraviolet in mice.
作者
邱勰
雷铁池
韩冰玉
姚云竹
廖志凯
胡双海
QIU Xie;LEI Tiechi;HAN Bingyu;YAO Yunzhu;LIAO Zhikai;HU Shuanghai(Department of Dermatology,Renmin Hospital of Wuhan University,Wuhan 430060,China)
出处
《中国麻风皮肤病杂志》
2021年第7期413-418,共6页
China Journal of Leprosy and Skin Diseases
基金
国家自然科学基金(编号:81972919)。
