摘要
目的研究益肾补骨汤对去卵巢大鼠骨代谢的调节作用。方法取51只大鼠建立去卵巢大鼠模型,另取10只大鼠设置为假手术组。将建模成功的大鼠随机分为尼尔雌醇组、高剂量益肾补骨汤组、中剂量益肾补骨汤组、低剂量益肾补骨汤组及空白模型组,每组各10只,灌胃治疗。对比各组大鼠股骨、胫骨骨密度,骨代谢指标;观察骨组织形态学变化;检测大鼠胫骨组织中基因和蛋白表达。结果模型组骨密度均较正常组下降,血清骨形成指标均下降、骨吸收指标均升高,胫骨组织病理改变严重,且骨形成蛋白-2(BMP-2)、Smad-1/5/8、Runt相关转录因子2(Runx 2)、骨保护素(OPG)mRNA和蛋白表达水平及p-Smad1/5/8水平均下降,而核因子κB受体活化因子配体(RANKL)、核因子κB受体活化因子(RANK)mRNA和蛋白表达水平均升高,尼尔雌醇组和益肾补骨汤三个剂量组对上述指标均有改善作用。益肾补骨汤的作用效果呈剂量依赖性,且益肾补骨汤中剂量作用与尼尔雌醇组接近,其余每两组间对比差异均有统计学意义(P<0.05)。结论益肾补骨汤可提高去卵巢大鼠骨密度,促进骨形成,减少骨吸收,推侧其机制可能与调控BMP/Smads通路、OPG/RANKL/RANK通路有关。
Objective To study the regulating effect of Yishen Bugu Decoction on bone metabolism in the ovariectomized rats.Methods 51 rats were selected to establish ovariectomized rat models,and another 10 rats were selected as sham operation group,and the rats with successful modeling were randomly divided into nylestriol group,high-dose Yishenbugu decoction group,medium dose Yishenbugu decoction group,low-dose Yishenbugu decoction group and blank model group,10 rats in each group,and all the rats were treated by gavage.Bone mineral density(BMD)of femur and tibia and bone metabolism indexes were compared.The morphological changes of bone tissue were observed,and gene and protein expressions in rat tibia tissues were detected.ResultsCompared with the normal group,the bone mineral density of the model group decreased,the serum bone formation indexes decreased,the bone resorption indexes increased,and the pathological changes of tibia were serious,and the mRNA and protein expression levels of bone morphogenetic protein-2(BMP-2),smad-1/5/8,Runt related transcription factor 2(Runx 2),osteoprotegerin(OPG)mRNA and protein levels and p-smad1/5/8 level decreased on average,and the mRNA and protein expressions of nuclear factorκB receptor activator ligand(RANKL)and nuclear factorκB receptor activator factor(RANK)were increased,while nylestriol group and Yishenbugu decoction three dose groups improved the above indexes.The effect of Yishenbugu decoction was dose-dependent,and the middle dose effect of Yishenbugu decoction was close to those of nilestriol group,and the differences between each other two groups were statistically significant(P<0.05).Conclusion Yishenbugu decoction can increase BMD,promote bone formation and reduce bone resorption in ovariectomized rats,and the mechanism may be related to the regulation of BMP/Smads pathway and OPG/RANKL/RANK pathway.
作者
刘雯
侯晨辉
李江
LIU Wen;HOU Chenhui;LI Jiang(Nursing College of Zhengzhou Railway Vocational and Technical College, Zhengzhou 451460, China;Henan Provincial Engineering Research Center of Natural Drug Extraction and Medical Technology Application, Zhengzhou 451460, China)
出处
《中国骨质疏松杂志》
CAS
CSCD
北大核心
2021年第6期788-792,共5页
Chinese Journal of Osteoporosis
基金
河南省科技厅重点研发与推广专项(202102310088)
河南省高等学校重点科研项目(20A320086)。
关键词
益肾补骨汤
去卵巢大鼠
骨形成
骨吸收
Yishenbugu decoction
ovariectomized rats
bone formation
bone resorption
