摘要
目的用高效液相色谱(HPLC)测定叶氏消渴方降糖颗粒剂中梓醇、没食子酸、芍药苷、丹皮酚、知母皂苷BⅡ及麦冬皂苷D的含量。方法采用HPLC-DAD测定叶氏消渴方降糖颗粒剂中梓醇、没食子酸、芍药苷、丹皮酚含量,色谱柱:Inertsil ODS-3(4.6 mm×250 mm,5μm);流动相:0.5%磷酸水溶液-乙腈等度洗脱(体积比92∶8);柱温:25℃;流速为0.8 mL·min^(-1);检测波长为210 nm;进样量10μL。采用HPLC-ELSD测定叶氏消渴方降糖颗粒剂中知母皂苷BⅡ、麦冬皂苷D含量,色谱柱:Lichrospher 5HE C_(18)(4.6 mm×250 mm,5μm);流动相:乙腈-水等度洗脱(体积比25∶75);柱温30℃,流速:1.0 mL·min^(-1),进样量10μL;ELSD条件:Alltech 3300蒸发光检测器;漂移管温度80℃,气体流量2.0 L·min^(-1),增益为8。结果上述6种成分在各自范围内线性关系良好,r均大于0.999;平均加样回收率在97.59%~100.97%(n=9),RSD值在0.14%~2.05%。结论所建立的梓醇、没食子酸、芍药苷、丹皮酚、知母皂苷BⅡ及麦冬皂苷D含量测定方法简便、准确、重复性好,可用于叶氏消渴方降糖颗粒剂主要成分含量测定。
Objective:To determine the content of six bioactive compounds,including catalpol,gallic acid,paeoniflorin,paeonol,timosaponin BⅡ,and ophiopogonin D in their hypoglycemic effects from Yeshixiaokefanggranules by HPLC.Methods:Simultaneous determinations of catalpol,gallic acid,paeoniflorin and paeonol were carried out by HPLC-DAD,with the Inertsil ODS-3(4.6 mm×250 mm,5μm)column,the mobile phase consisted of 0.5%phosphoric acid aqueous solution and water(92∶8),injection volume 10μL,column temperature,detection wavelength and flow rate set at 30℃,210 nm and 0.8 mL·min^(-1),respectively.HPLC-ELSD was adopted to determine the content of timosaponin BⅡ,ophiopogonin D with the Lichrospher 5HE C_(18)(4.6 mm×250 mm,5μm)column,the mobile phase of acetonitrile-water(25∶75),flow rate of 1.0 mL·min^(-1),and Alltech ELSD 3300(Eva:80℃,gas flow:2.0 L·min^(-1)).Results:There was a strong linear relationship among the content of catalpol,gallic acid paeoniflorin,paeonol,timosaponin BⅡand ophiopogonin D(r>0.999).The average recoveries ranged from 97.59%~100.97%(n=9),and RSD ranged from 0.14%~2.05%.Conclusion:Our present method displayed good repeatability and high precision,and thus can be applied for the analysis of the six constitutes of Yeshixiaokefang granules for their hypoglycemic effect.
作者
杨力龙
龚志强
霍宇
梁天坚
汪磊
李思梅
刘方蓉
YANG Li-long;GONG Zhi-qiang;HUO Yu;LIANG Tian-jian;WANG Lei;LI Si-mei;LIU Fang-rong(First Affiliated Hospital, Guangxi University of Chinese Medicine, Nanning 530023, China;Faculty of Chinese Medicine Science, Guangxi University of Chinese Medicine, Nanning 530222, China)
出处
《湖南师范大学自然科学学报》
CAS
北大核心
2021年第3期74-80,共7页
Journal of Natural Science of Hunan Normal University
基金
广西教育厅高校中青年教师基础能力提升项目(KY2015YB513,KY2016YB829)
广西壮族自治区大学生创新创业项目(201713643037)
广西中医药大学大学生科研训练项目(2017DXS49)。
