摘要
目的:分析探讨滋肾排石汤对结石大鼠尿液pH值、钙水平和草酸水平的影响。方法:72只SD大鼠随机分为滋肾排石汤组、结石组与对照组,每组各24只。对照组大鼠标准饮食,自由饮水;其余两组标准饮食,1%乙二醇自由饮水,每日清晨采用2%氯化铵灌胃,每只2 mL,连续实施4周。滋肾排石汤组给药剂量5.4 g/kg,每100 g灌胃2 mL,连续实施4周。对比各组间大鼠双肾成石程度、尿pH、尿钙与尿草酸水平,氧化应激指标超氧化物歧化酶(SOD)、丙二醛(MDA)含量,炎性相关蛋白转化生长因子-β(TGF-β)和骨桥蛋白(OPN)阳性率。结果:Pearsonχ^(2)检验结果显示,双肾成石率由高到低依次为结石组>滋肾排石汤组组>对照组(P<0.01)。Mann-Whitney U检验结果显示:各组间尿pH比较差异均无统计学意义(P>0.05),滋肾排石汤组尿钙与尿草酸水平均低于结石组(P<0.05)。与结石组相比,滋肾排石汤组大鼠肾脏组织中MDA含量、TGF-β和OPN阳性率显著降低(P<0.05),SOD含量显著升高(P<0.05)。结论:滋肾排石汤可能通过改善氧化应激和炎性反应以降低尿钙与低尿草酸含量及双肾的成石率。
Objective:To analyze the effect of Zishen Paishi Decoction on urine pH value,calcium level and oxalic acid level in rats with calculi.Methods:72 SD rats were randomly divided into Zishen Paishi decoction group,stone group and control group,24 cases in each group.Rats in the control group were fed standard diet and drank freely,the other two groups were given standard diet,1%glycol free drinking water,2%ammonium chloride per morning,2 mL for each,for 4 weeks.Dose 5.4 g/kg,for Zishen PaiShi Decoction Per 100 g 2 mL,of gavage continuous implementation for 4 weeks.Comparison of renal lithogenesis,urinary pH,urinary calcium and urinary oxalic acid in rats,Oxidative stress indicator superoxide dismutase(SOD),malondialdehyde(MDA)content,positive rates of inflammatory-associated protein transforming growth factor-β(TGF-β)and osteopontin(OPN)were observed.Results:Pearsonχ^(2) test results showed that the rate of double kidney stone formation from high to low was in stone group>Zishen Pai Shi Tang group>control group(P<0.01).The results of Mann-Whitney U test showed that there was no significant difference in urine pH between the groups(P>0.05).The levels of urine calcium and urinary oxalic acid in Zishen PaiShi decoction group were significantly lower than those in stone group(P>0.05).Compared to the stone group,the positive rate of MDA content,TGF-βand OPN in kidney tissue of Zishen PaiShi Tang group decreased significantly(P<0.05),the SOD content increased significantly(P<0.05).Conclusion:Zishen PaiShi Decoction can significantly reduce the content of calcium and hypuria oxalic acid in both kidneys to reduce the rate of stone formation,may be related to its improvement of oxidative stress and inflammatory response.
作者
马志强
左岩
张翼
MA Zhi-qiang;ZUO Yan;ZHANG Yi(Department of Urology Surgery,Shijiazhuang Third Hospital,Shijiazhuang 050000,Hebei,China)
出处
《川北医学院学报》
CAS
2021年第6期696-699,共4页
Journal of North Sichuan Medical College
基金
河北省卫生和计划生育委员会科研基金项目(20170992)。
关键词
滋肾排石汤
大鼠
草酸钙结石
氧化应激
炎性反应
Zishen PaiShi Tang
Rats
Calcium Oxalate Stone
Oxidative Stress
Inflammatory Response
