摘要
目的:确定牛肝菌多酚的细胞抗氧化活性评价模型。方法:基于人结肠癌细胞Caco-2、人肝细胞L02和人肝癌细胞HepG23种细胞,检测黄牛肝菌多酚的细胞抗氧化活性,确定适宜的细胞抗氧化评价模型。采用适宜细胞模型测定红牛肝菌、黄牛肝菌和黑牛肝菌多酚的细胞抗氧化活性,并与牛肝菌的多酚提取率、氧化自由基吸收能力进行相关性分析,验证方法的有效性和准确性。结果:3种细胞模型均能检出黄牛肝菌多酚的细胞抗氧化活性,而在检测多酚浓度范围、灵敏度方面存在差异,Caco-2、L02和HepG2模型检出限分别为0.025,0.1,0.2 g黄牛肝菌提取的多酚,其中Caco-2检出限最低,明显优于L02和HepG2模型。基于Caco-2细胞模型测定3种牛肝菌多酚的抗氧化能力,与牛肝菌的多酚提取率、ORAC相关系数分别为0.9662和0.9156,为显著性相关,Caco-2细胞模型有效、准确。结论:Caco-2细胞模型更适用于牛肝菌多酚细胞抗氧化活性检测,可与ORAC等体外化学评价方法联合评价牛肝菌多酚的抗氧化活性。
Objective:To determine the evaluation model of cellular antioxidant activity for boletus polyphenols.Methods:Based on three different cells,human colon cancer cell Caco-2,human liver cell L02 and human liver cancer cell HepG2,the antioxidative activity of the boletus polyphenols was detected,and a suitable cellular antioxidant evaluation model was determined.Using a suitable cell model,cellular antioxidant activity of polyphenols from Boletus rubeus,Boletus auripes,Boletus nigricans was determined,and analysis correlation with the polyphenol extraction rate and oxygen radical absorbance capacity of boletus to verify the effectiveness and accuracy.Results:Three cell models could detect the cellular antioxidant activity from Boletus auripes polyphenols,but there were differences in the range and sensitivity of the detection.The detection limits of Caco-2,L02 and HepG2 models were 0.025,0.1,0.2 g polyphenols which were extracted from Boletus auripes respectively.Among them,detection limit of Caco-2 was the lowest and significantly better than L02 and HepG2 models.The Caco-2 cell model was used to determine the antioxidant capacity of three boletus polyphenols.The polyphenol extraction rate and ORAC correlation coefficients were 0.9662 and 0.9156 respectively.By significant correlation,the Caco-2 cell model was effective and accurate.Conclusion:The Caco-2 cell model was more suitable for detecting the cellular antioxidant activity of boletus polyphenols.It can be used to evaluate the antioxidant activity of boletus polyphenols by combine with ORAC and other chemical evaluation methods in vitro.
作者
王晶波
杨倬
秦文
王丽媛
陈曦
卓勤
宫照龙
沈葹
Wang Jingbo;Yang Zhuo;Qin Wen;Wang Liyuan;Chen Xi;Zhuo Qin;Gong Zhaolong;Shen Shi(Key Laboratory of Trace Element Nutrition of National Health Commission(NHC),National Institute for Nutrition and Health,Chinese Center for Disease Control and Prevention,Beijing 100050)
出处
《中国食品学报》
EI
CAS
CSCD
北大核心
2021年第6期273-279,共7页
Journal of Chinese Institute Of Food Science and Technology
关键词
细胞抗氧化
牛肝菌多酚
评价模型比较
cellular antioxidant activity
boletus polyphenols
comparison of evaluation models
