摘要
目的:研究在解脲脲原体(UU)检测中实时荧光核酸恒温扩增技术(SAT)和液体培养法的诊断价值。方法:收集141例2018年6月~2019年6月间前来某院就诊的疑似解脲脲原体感染的患者为研究对象,所有患者均采集2份拭子样本,其中1份拭子样本进行SAT检测,1分拭子样本进行液体培养,比较两种检测结果。结果:SAT法检测阳性率低于液体培养法(P<0.05);SAT法检测灵敏度高于液体培养法(P<0.05),特异度高于液体培养法(P<0.05)。结论:解脲脲原体(UU)检测中SAT法比液体培养法灵敏度和特异度高,而且低污染、时间短、样本采集方便、反应稳定,检测结果更加准确。
Objective:To study the diagnostic value of real-time fluorescent nucleic acid amplification(SAT)and liquid culture in the detection of ureaplasma urealyticum(UU).Methods:A total of 141 patients with suspected ureaplasma urealyzae infection who came to a hospital between June 2018 and June 2019 were collected as study subjects.Two swab samples were collected from all patients,including 1 swab sample for SAT test and 1 swab sample for liquid culture.The two test results were compared.Results:The positive rate of SAT was lower than that of liquid culture(P<0.05).The sensitivity of SAT method was higher than that of liquid culture method(P<0.05),and the specificity was higher than that of liquid culture method(P<0.05).Conclusion:In the detection of ureaplasma urealyticum(UU),the SAT method has higher sensitivity and specificity than the liquid culture method,with low pollution,short time,convenient sample collection,stable reaction,and more accurate detection results.
作者
郑敬业
Zheng Jingye(Departmentof Clinical Laboratory,Yanling County People's Hospital,Henan Province,Xuchang 461200)
出处
《数理医药学杂志》
CAS
2021年第7期982-984,共3页
Journal of Mathematical Medicine
关键词
实时荧光核酸恒温扩增技术
液体培养法
解脲脲原体
real-time fluorescent nucleic acid constant temperature amplification technology
liquid culture method
ureaplasma urealyticum
