摘要
结合损伤评分和优化的染料穿透实验,以离体皮肤为受试材料,建立了一种洗涤和消毒产品皮肤腐蚀性的快速分类方法。通过构建和考察染药时间-观察时间-损伤效应曲面,确定了损伤评分的判定条件。比较受试皮肤在不同提取条件下的染料提取效果,优化了染料穿透实验的关键条件,采用超声波提取的方法缩短了实验时间,提高了实验效率。利用16个参考物质对该方法进行验证,获得一致性良好的结果(Kappa=0.826,P=0.000),腐蚀性判别的灵敏度、特异度和准确度分别达到100%,85.7%和93.8%,腐蚀性分类的准确度达到87.5%。该方法简便、高效、节约成本和时间且符合动物伦理3R原则,适用于大量样品的腐蚀性快速筛查和分类,对加强化学品的安全管理,预防和减少化学品事故具有重要意义。
A combined method of in-vitro skin damage grading and dye penetration was established for rapid classification of skin corrosion of detergents and disinfectants.By constructing and investigating the surface of exposure time-observation time-skin reaction grade,the criteria base on the damage grading were determined.The dye extraction effect of the tested skin under different extraction conditions was compared,and the key condition of the dye penetration experiment was optimized.The utilizing of ultrasonic extraction shortens the experiment time and improves the efficiency of the experiment.The method is verified with 16 reference substances,and the results are well consistent(Kappa=0.826,P=0.000).The sensitivity,specificity and accuracy of the corrosive judgment are 100%,85.7%and 93.8%,respectively,and the accuracy of the corrosive classification is 87.5%.The method is convenient,rapid,efficient and low-cost,and conforms to 3 Rs principle of animal research ethics.Thus,it is suitable for rapid classification of a large number of samples to enhance the safety management of chemicals and prevent the chemical accidents.
作者
潘叙恩
何志荣
卢章顺
杨光
钟岸
黄志鹏
Xuen Pan;Zhirong He;Zhangshun Lu;Guang Yang;An Zhong;Zhipeng Huang(Institute of Analysis,Guangdong Academy of Sciences(China National Analytical Center,Guangzhou),Guangzhou,Guangdong 510070,China;Guangdong Provincial Key Laboratory of Emergency Test for Dangerous Chemicals,Guangzhou,Guangdong 510070,China;Guangdong Provincial Public Laboratory of Analysis and Testing Technology,Guangzhou,Guangdong 510070,China)
出处
《日用化学工业》
CAS
北大核心
2021年第7期639-647,共9页
China Surfactant Detergent & Cosmetics
基金
广东省科学院发展专项资金项目(2019GDASYL-0105020)。
关键词
皮肤腐蚀性
快速分类
离体皮肤
染料穿透
损伤评分
skin corrosion
rapid classification
in-vitro skin
dye penetration
damage grading
