摘要
【目的】土壤微生物群落结构对外界环境变化敏感,不同的植被类型、气候类型、经营管理措施、土地利用方式、地形梯度等显著影响土壤微生物群落结构。本研究采用新的采伐模式对毛竹Phyllostachys edulis林进行带状采伐(采伐宽度为3、6、9、12 m,长20 m的采伐带),探究带状采伐措施对毛竹林土壤细菌群落物种组成、多样性及系统发育学特征的影响,以评估毛竹林带状采伐经营技术的适用性与合理配置方式。【方法】利用Illumina HiSeq 2500测序平台,采用16S rRNA的V3-V4区段引物对土壤细菌进行高通量测序。【结果】毛竹林带状采伐后,土壤放线菌门(Actinobacteria)的相对丰度在3 m采伐带显著增加(P<0.05),其余细菌类群无显著变化;3、9 m采伐带的Ace多度指数显著大于保留带(P<0.05),细菌Shannon和Simpson多样性指数无显著变化;9 m采伐带改变了细菌群落结构,且群落结构最相似;3、6 m的全磷含量显著升高(P<0.05),其他养分指标及酶活性无显著影响;经mantel检验,确定门水平细菌群落主要受全氮(R=0.31,P=0.046)、全磷(R=0.19,P=0.040)、C:P(R=0.40,P=0.026)、N:P(R=0.29,P=0.038)的驱动;细菌的系统发育多样性指数(PD)在3 m采伐带显著升高,净种间亲缘关系距离指数(NRI)/净最近种间亲缘关系指数(NTI)大于0,土壤细菌群落为系统发育聚集(phylogenetic clustering),细菌群落结构的维持机制符合生态位理论,环境因子决定了微生物的群落结构。【结论】毛竹林经带状采伐后短期恢复过程中,所设置的采伐带宽还未达到土壤细菌群落的中度干扰程度,据已有研究成果,若短期内使用带状采伐经营毛竹林可设置9 m采伐带宽,土壤细菌群落的恢复力和抵抗力较强,稳定性较高。
【Objective】The structure of soil microbial community is sensitive to the change of external environment,and different vegetation types,climate types,management measures,land use patterns and terrain gradient have significant influence on the structure of soil microbial community.This research adopts the new logging mode of moso bamboo(Phyllostachys edulis)to strip cutting,the strip width is 3,6,9 and 12 m,20 m of the cutting length,to explore the strip cutting measures of moso bamboo forest on soil bacteria community species composition,diversity,and phylogenetics characteristics,to assess the applicability of the moso bamboo forest strip cutting operation technology and reasonable configuration mode.【Method】Based on Illumina HiSeq 2500 sequencing platform,16 s rRNA V3-V4 sections primer was used to high-throughput sequencing.【Result】After strip clear-cutting,the relative abundance of soil Actinobacteria increased significantly in the 3 m cutting zone(P<0.05).The Ace abundance index of bacteria in 3 m and 9 m cutting zone was significantly higher than that of reserved zone(P<0.05),and the diversity index of bacterial Shannon and Simpson had no significant change.The 9 m cutting strip changed the bacterial community structure,and the community structure was the most similar.The total phosphorus content of 3 m and 6 m cutting strip increased significantly(P<0.05),while other nutrient indexes and enzyme activity had no significant effect.By mantel test,it was determined that the bacterial community at phylum level was mainly driven by total nitrogen(R=0.31,P=0.046),total phosphorus(R=0.19,P=0.040),C:P(R=0.40,P=0.026),and N:P(R=0.29,P=0.038).Bacterial phylogenetic diversity(PD)rise significantly in 3 m cutting strip,net relatedness index(NRI)ornearest taxon index(NTI)greater than zero,soil bacteria community tend to the phylogenetic clustering,maintaining mechanism of bacterial community structure conforms to the niche theory,environmental factor determine the microbial community structure.In the short-term recovery process of bamboo forest after strip clear-cutting,the cutting strip width has not yet reached the moderate interference degree of the soil bacterial community.According to the existing research results,if the strip clear-cutting is used in the short-term management of bamboo forest,9 m cutting strip width can be set,and the soil bacterial community has high stability.
作者
吴昌明
范少辉
冯云
申景昕
刘广路
WU Changming;FAN Shaohui;FENG Yun;SHEN Jingxin;LIU Guanglu(Key Laboratory of Science and Technology of Bamboo and Rattan of State,International Centre for Bamboo and Rattan,Beijing 100012,China)
出处
《中南林业科技大学学报》
CAS
CSCD
北大核心
2021年第7期42-51,共10页
Journal of Central South University of Forestry & Technology
基金
国际竹藤中心基本业务专项“毛竹林高产低耗培育技术模式下土壤微环境特征研究”(1632019005)
“十三五”重点研发计划项目“材用竹林高效培育及规模经营技术集成示范”(2016YFD0600902)
“材用竹林高效培育与监测技术”(2018YFD0600103)。
关键词
带状采伐
毛竹
土壤细菌
群落结构
多样性
strip cutting
moso bamboo
soil bacteria
community structure
diversity
