摘要
目的本研究旨在探索铅染毒是否会导致斑马鱼焦虑的发生,并对其机制进行初步探索。方法于2020年5月,收集受精后4 h(4hpf)的斑马鱼胚胎,以E3培养液作为对照组,不同的铅染毒浓度(6、12、24、48μmol/L)作为染毒组,染毒时间为140 h。计算144 hpf斑马鱼的胚胎死亡率、胚胎孵化率和幼鱼畸形率;观察144 hpf幼鱼的行为学变化(运动速度、移动距离、活跃度、绝对转角、光照惊恐反应、黑暗逃避反应和趋触性)。并且检测斑马鱼幼鱼头部的活性氧(ROS)、丙二醛(MDA)水平。用ELISA试剂盒检测与焦虑相关的神经递质5-羟色胺(5-HT)、多巴胺(DA)、去甲肾上腺素(NA)和促肾上腺皮质激素释放激素(CRH)的表达量。结果12、24和48μmol/L铅染毒组斑马鱼胚胎死亡率高于对照组,胚胎孵化率低于对照组,24和48μmol/L铅染毒组斑马鱼幼鱼畸形率高于对照组(P<0.01);24和48μmol/L铅染毒组幼鱼运动速度、活跃度和趋触性低于对照组,绝对转角高于对照组,48μmol/L铅染毒组幼鱼移动距离和黑暗逃避反应低于对照组,12、24和48μmol/L铅染毒组幼鱼的光照惊恐反应低于对照组(P<0.05)。24和48μmol/L铅染毒组幼鱼头部ROS水平和MDA浓度高于对照组(P<0.05);12、24和48μmol/L铅染毒组幼鱼头部NA和DA的含量低于对照组,24和48μmol/L铅染毒组幼鱼头部5-HT和CRH浓度高于对照组(P<0.05)。结论铅对斑马鱼有一定的胚胎发育毒性,且可导致斑马鱼发生焦虑样神经行为、神经递质改变以及氧化应激的发生。
Objective This study aims to explore whether lead exposure could cause anxiety of zebrafish larvae,and to explore its mechanism.Methods In May 2020,zebrafish embryos 4 hours after fertilization(4 hpf)were collected,E3 culture medium was used as the control group,and different lead exposure concentrations(6,12,24,48μmol/L)as the poisoned group during the time was 140 h.The mortalities,hatching rates and deformity rates were calculated,the behavioral changes of 144 hpf larvae were observed(moving speed,moving distance,activity,absolute turn angle,the light-evoked startle response,darkness-evoked escape response and thigmotaxis).The levels of reactive oxygen species(ROS)and malondialdehyde(MDA)were detected in zebrafish larvae in the heads.ELISA kits were used to detect the expression of 5-hydroxytryptamine(5-HT),dopamine(DA),noradrenaline(NA)and corticotropin-releasing hormone(CRH)related to anxiety.Results The mortalities rates of zebrafish embryos in the lead exposure concentration of 12,24 and 48μmol/L were higher than the control group,and the embryo hatching rates were lower than control group.The deformity rates of zebrafish larvae in the lead exposure concentration of 24 and 48μmol/L were higher than control group(P<0.01).The movement speed,activity and thigmotaxis of zebrafish larvae in the lead exposure concentration of 24 and 48μmol/L were significantly lower than control group,the absolute turn angle were higher than control group(P<0.01).The moving distance and darkness-evoked escape response of zebrafish larvae in the lead exposure concentration of 48μmol/L group were lower than control group(P<0.05).The light-evoked startle response of zebrafish larvae in the lead exposure concentration of 12,24 and 48μmol/L groups were lower than control group(P<0.05).The ROS levels and MDA concentrations in the heads of larvae in the lead exposure concentration of 24 and 48μmol/L were higher than control group(P<0.05).The levels of NA and DA in the heads of the larvae in the lead exposure groups of 12,24 and 48μmol/L were lower than control group,and the levels of 5-HT and CRH in the heads of the larvae in the lead exposure groups of 24 and 48μmol/L were higher than control group(P<0.05).Conclusion Lead exposure is toxic to zebrafish embryonic development and may cause anxiety-like neurobehavior changes and oxidative stress in zebrafish.
作者
郑晓俊
王婷
田亚
胡志强
尚楠
Zheng Xiaojun;Wang Ting;Tian Ya;Hu Zhiqiang;Shang Nan(Department of Pharmacy,First Hospital of Shanxi Medical University,Taiyuan 030001,China;School of Pharmacy,Shanxi Medical University,Taiyuan 030001,China)
出处
《中华劳动卫生职业病杂志》
CAS
CSCD
北大核心
2021年第6期407-411,共5页
Chinese Journal of Industrial Hygiene and Occupational Diseases
关键词
铅
斑马鱼
运动能力
焦虑样行为
Lead
Zebrafish
Movement ability
Anxiety-like behavior
