摘要
为了筛选出对貉源耐药性大肠杆菌菌株具有明显抑制和消除耐药性作用的单味中药,本研究采用平板琼脂打孔法和试管二倍稀释法测定5味中药水煎剂对3株大肠杆菌的抑菌圈直径和最小抑菌浓度(MIC),观察比较5味中药的抗菌活性;将各分离株在5味中药水煎剂中培养,采用纸片扩散法(K-B法)测定大肠杆菌耐药性的变化;采用PCR法检测五味子、黄连和诃子分别作用大肠杆菌后耐药基因的变化;并采用琼脂平板培养计数法筛选中药作用后耐药性被消除的菌落。结果显示:苏木、诃子、秦皮、五味子和黄连对大肠杆菌均有一定的抑菌作用,抑菌圈的直径≥14 mm,其中抑菌效果较强的是五味子、诃子和秦皮,其MIC为31.25 mg/mL~62.50 mg/mL;5味中药对貉大肠杆菌的耐药性均有一定的消除作用,消除率为2.8%~11.6%,其中秦皮的平均消除率最高为8.8%;五味子、黄连和诃子作用后大肠杆菌丢失2~3个耐药基因,并且在中药作用后大肠杆菌恢复了对头孢拉定等抗菌药的敏感性。结果表明,5味中药对貉大肠杆菌具有明显的抑菌效果,并且具有消除耐药基因和逆转耐药性的作用,其中效果较强的是五味子、诃子和秦皮,可作为抗菌药的替代品应用于貉养殖业,不仅对防治貉大肠杆菌病起到一定作用,同时也能降低貉大肠杆菌的耐药性,对解决貉养殖业的貉大肠杆菌病具有重要的意义。
The purpose of this study was to screen for the traditional Chinese medicines that inhibit E. coli isolated from raccoon dogs and eliminate drug resistance of these E. coli isolates. The minimum inhibitory concentrations(MICs) and inhibition zone diameter of 5 traditional Chinese medicines on 3 E. coli isolates were determined by cylinder-plate method and 2-fold dilution method. The isolates were cultivated respectively in decoction of 5 traditional Chinese medicines to test the restoring sensitivity of antimicrobial agents using the K-B method. The PCR method was used to detect the changes of drug resistance genes of E. coli isolates after treatment with Schisandra, Sophora flavescens and Terminalia chebula. And agar plate culture counting method was used to identify the isolates with eliminated drug resistance. The results showed that Sappan lignum, Terminalia chebula, Cortex Fraxini, Schisandra and Sophora flavescens had different inhibitory effects on the E. coli isolates, and diameters of bacteriostasis circles were larger than 14 mm. Schisandra, Terminalia chebula and Cortex Fraxini had the strongest antibacterial effect, with the MICs ranging from 31.25 mg/m L to 62.50 mg/m L. The drug resistance was eliminated from 2.8% to 11.6% in the isolates when treated with 5 traditional Chinese medicines, and Cortex Fraxini had the highest elimination rate of 9.33%. Two to 3 drug resistance genes were lost in E. coli after the interaction with Schisandra, Sophora flavescens and Terminalia chebula. And traditional Chinese medicine treatment recovered the susceptibility of E. coli to cefradine and other antibiotics. In conclusion, 5 traditional Chinese medicines have obvious antibacterial effects on E. coli from raccoon dogs, and have the effects of eliminating drug resistance genes and drug resistance. Schisandra, Terminalia chebula and Cortex Fraxini can be used as substitutes for antibacterial drugs in the raccoon breeding industry, which not only plays a role in preventing and controlling E. coli, but also reduces the drug resistance of E. coli, which is of great significance for raccoon breeding to solve the E. coli issue.
作者
王婧文
谭贻鸿
高昊天
张东林
史秋梅
高桂生
高光平
WANG Jing-wen;TAN Yi-hong;GAO Hao-tian;ZHANG Dong-lin;SHI Qiu-mei;GAO Gui-sheng;GAO Guang-ping(Hebei Normal University of Science&Technology/Key Laboratory of Preventive Veterinary Medicine of Hebei Province,Qinhuangdao 066004,China;Beijing University of Chinese Medicine DongFang College,Langfang 065001,China)
出处
《中国预防兽医学报》
CAS
CSCD
北大核心
2021年第4期370-375,共6页
Chinese Journal of Preventive Veterinary Medicine
基金
秦皇岛市科技局项目(201903B007)
河北省第三批巨人计划-中草药防治畜禽水产养殖动物传染病科研创新团队
河北科技师范学院科学研究基金。
关键词
貉
大肠杆菌
中药
抑菌效果
耐药性
raccoon dog
E.coli
traditional Chinese medicines
inhibitory effect
drug resistance
