摘要
目的评价Geno-type MTBDRplus分子线性探针杂交技术显色法(以下简称杂交酶显色法)在结核病快速诊断及检测异烟肼(INH)和利福平(RIF)药物敏感中的应用。方法选取2016年1月~2019年12月的临床确诊结核病患者的临床标本413例,同时采用BD 960液体培养法和杂交酶显色法进行结核分枝杆菌检测,并对其中108株菌株进行异烟肼和利福平药敏检测,比较两种方法的一致性。结果杂交酶显色法测得结核分枝杆菌358例(86.68%),BD 960液体培养法测得结核分枝杆菌376例(91.04%);两种方法结果相符349例(84.50%)。108株菌株中杂交酶显色法和BD 960液体培养法分别检出异烟肼敏感87株(80.56%)和88株(81.48%),检出利福平敏感96株(88.89%)和94株(87.04%)。两种方法对结核分枝杆菌的鉴定及异烟肼、利福平药物敏感检测具有高度一致性。结论与BD 960液体培养法相比,杂交酶显色法检测结核分枝杆菌及异烟肼和利福平药敏更为快速有效。
Objective To evaluate the utility of the Geno-type MTBDRplusmolecular linear probe hybridization coloration assay(hereafter referred to asline probeassay)for rapid screening of tuberculosis and drug sensitivity to isoniazid(INH)and rifampicin(RIF).Methods 413 cases of clinically diagnosed tuberculosis from January 2016 to December 2019 were included.The BD 960 liquid culture method and the line probeassay were used to detect Mycobacterium tuberculosis.Among them,108 strains were also tested for INH and RIF drug sensitivity.The consistency of the two methods was compared.Results 358 cases of Mycobacterium tuberculosis were detected by line probeassay and 376 cases by BD 960 liquid culture method.349 cases(84.5%)were identified by the two methods.Among 108 strains,89 strains were sensitive to INH,98 strains were sensitive to RIF,and 77 strains were sensitive to INH and RIF.In BD 960 liquid culture,85 strains were INH sensitive,93 strains were RIF sensitive,81 strains were INH and RIF sensitive.The coincidence rate of INH and RIF was 67.59%and 85.191%respectively.The results of identification of Mycobacterium tuberculosis and analysis of INH and RIF drug sensitivity showed that the two methods were consistent.Conclusion Compared with the BD 960 liquid culture method,the line probeassay provides a more rapid and effective method for the clinical diagnosis of tuberculosis and INH and RIF drug-resistance,which may provide a basis for the development of targeted treatment for patients.
作者
李津
李昌锦
杨洁
黄婷
樊金燕
LI-jin;LI Chang-jin;YANG Jie;HUANG Ting;FAN Jin-yan(Department of Tuberculosis,the 900 Hospital of Joint Logistics Support Force of Chinese People’s Liberation Army,Fuzhou 350003,China;Department of Clinical Laboratory,the 900 Hospital of Joint Logistics Support Force of Chinese People’s Liberation Army,Fuzhou 350003,China)
出处
《现代检验医学杂志》
CAS
2021年第4期119-121,共3页
Journal of Modern Laboratory Medicine
关键词
线性杂交酶显色法
结核分枝杆菌
药物敏感性
linear hybridization enzyme coloration assay
Mycobacterium tuberculosis
drug sensitivity
