摘要
目的探讨锌指样转录因子4(KLF4)、乳酸脱氢酶(LDH)、P53与弥漫性大B细胞淋巴瘤(DLBCL)标准治疗反应性关系及联合预测价值。方法选取2017年1月至2020年3月本院收治的62例DLBCL患者,根据治疗反应性分为缓解组(n=42)、非缓解组(n=20),比较两组基线资料、KLF4mRNA、血清LDH水平、P53基因缺失率,采用Pearson分析KLF4 mRNA、LDH与国际预后指数(IPI)评分关系,采用多因素Logistic回归方程分析治疗反应性的相关影响因素,采用受试者工作特征曲线(ROC)及曲线下面积(AUC)分析各指标预测治疗反应性的价值。结果缓解组Ann Arbor分期、IPI评分与非缓解组比较,差异有统计学意义(P<0.05);缓解组KLF4 mRNA高于非缓解组,LDH、P53基因缺失率低于非缓解组,差异有统计学意义(P<0.05);KLF4 mRNA与IPI评分呈负相关(r=-0.611,P<0.001),LDH与IPI评分呈正相关(r=0.728,P<0.001);将Ann Arbor分期、IPI评分控制后,KLF4 mRNA、LDH、P53基因缺失仍与治疗反应性相关(P<0.05);KLF4 mRNA、LDH、P53基因预测缓解的AUC为0.829、0.832、0.614,KLF4 mRNA+LDH+P53基因预测缓解的AUC为0.962(P<0.05)。结论KLF4 mRNA、LDH、P53基因缺失与DLBCL标准治疗反应性及预后有关,在缺乏有效手段时,可作为预测缓解的标志物为临床提供重要参考信息。
Objective To explore the relationship between zinc finger⁃like transcription factor 4(KLF4),lactate dehydrogenase(LDH),P53 and the standard treatment response of diffuse large B⁃cell lym⁃phoma(DLBCL)and the combined predictive value.Methods A total of 62 patients with DLBCL admitted to our hospital from January 2017 to March 2020 were divided into remission group(n=42)and non⁃remission group(n=20)according to treatment response.The baseline data and KLF4 of 2 groups were compared.mRNA,serum LDH level,P53 gene deletion rate,Pearson analysis of the relationship between KLF4 mRNA,LDH and the International Prognostic Index(IPI)score,multivariate Logistic regression equation to analyze the relevant influencing factors of treatment response,and receiver operating characteristic curve(ROC)and area under the curve(AUC)analyze the value of each index in predicting treatment response.Results The Ann Arbor staging and IPI score of the remission group were compared with the non⁃remission group,and the difference was statistically significant(P<0.05);the KLF4 mRNA of the remission group was higher than that of the non⁃remission group,and the LDH and P53 gene deletion rate was lower than that of the non ⁃ remission group. Statistical significance(P<0.05);KLF4 mRNA was negatively correlated with IPI score(r=-0.611,P<0.001),LDH was positively correlated with IPI score(r=0.728,P<0.001);Ann Arbor staging,IPI After scoring control,KLF4 mRNA,LDH,and P53 gene deletions are still related to treatment response(P<0.05);KLF4 mRNA,LDH,and P53 genes predict remission AUC of 0.829,0.832,0.614,and KLF4 mRNA + LDH + P53 gene prediction The remission AUC was 0.962 (P<0.05). Conclusion KLF4 mRNA,LDH,and P53 gene deletions are related to the responsiveness and prognosis of DLBCL standard treatments. In the absence of effective methods,they can be used as markers to predict remission and provide important clinical reference information.
作者
秦福丽
郭志强
李小雨
QIN Fuli;GUO Zhiqiang;LI Xiaoyu(Department of Hematology,Zhengzhou Central Hospital Affiliated to Zhengzhou University,Zhengzhou,Henan,China,450007)
出处
《分子诊断与治疗杂志》
2021年第7期1122-1125,共4页
Journal of Molecular Diagnostics and Therapy
基金
郑州大学校级教育教学改革研究与实践项目(2019ZZUJGLX353)。
