摘要
目的:探讨长基因间非编码RNA 00519(long intergene non-coding RNA 00519,LINC00519)调控miR-876-3p/高迁移率家族蛋白A1(high mobility group protein A1,HMGA1)轴在胃癌HGC-27细胞的增殖、凋亡、迁移和侵袭中的作用。方法:采用qPCR检测胃癌细胞HGC-27和胃黏膜上皮细胞GES-1中LINC00519的表达水平。将HGC-27细胞按转染处理分为si-NC、si-LINC00519、si-LINC00519+anti-miR-NC和si-LINC00519+anti-miR-876-3p组,采用集落形成实验检测细胞克隆形成能力,流式细胞术检测细胞凋亡和周期分布,Transwell实验检测细胞迁移和侵袭。双荧光素酶报告实验和qPCR验证LINC00519与miR-876-3p、miR-876-3p与HMGA1之间的相互作用。结果:HGC-27细胞中LINC00519表达较GES-1细胞显著升高(P<0.05),转染siRNA后si-LINC00519组HGC-27细胞中LINC00519的表达水平较si-NC组显著降低(t=47.294,P<0.01)。与si-NC组比较,si-LINC00519组HGC-27细胞克隆数、迁移侵袭数、S期细胞比例均显著降低(均P<0.01),凋亡率、G0/G1期细胞比例均显著升高(均P<0.01)。与si-LINC00519+anti-miR-NC组比较,si-LINC00519+anti-miR-876-3p组HGC-27细胞克隆数、迁移侵袭数、S期细胞比例升高(均P<0.01),凋亡率、G0/G1期细胞比例显著降低(均P<0.01)。LINC00519能够靶向负调控miR-876-3p的表达,miR-876-3p靶向负调控HMGA1的表达。结论:敲降LINC00519能够通过调控miR-876-3p/HMGA1轴抑制胃癌HGC-27细胞的增殖、迁移和侵袭,诱导细胞凋亡。
Objective:To investigate the effect of long intergene non-coding RNA 00519(LINC00519)on the proliferation,apoptosis,migration and invasion of gastric cancer HGC-27 cells by regulating the miR-876-3 p/high mobility group protein A1(HMGA1)axis.Methods:The expression levels of LINC00519 in gastric cancer HGC-27 cells and gastric mucosal epithelial GES-1 cells were detected by qPCR.HGC-27 cells were divided into si-NC,si-LINC00519,si-LINC00519+anti-miR-NC,and si-LINC00519+anti-miR-876-3 p groups according to transfection treatment.Colony formation test was applied to detect cell cloning ability;Flow cytometry was used to detect apoptosis and cell cycle distribution;Transwell assay was selected to measure cell migration and invasion.Dual luciferase reporter gene assay and qPCR were applied to confirm the interaction between LINC00519 and miR-876-3 p as well as between miR-876-3 p and HMGA1.Results:The expression of LINC00519 in HGC-27 cells was significantly higher than that in GES-1 cells(P<0.05).After siRNA transfection,the expression level of LINC00519 in HGC-27 cells of the si-LINC00519 group was significantly lower than that of the si-NC group(t=47.294,P<0.01).Compared with the si-NC group,the number of HGC-27 cell clones,the number of migrated and invaded cells,and proportion of S-phase cells in the si-LINC00519 group were significantly decreased(all P<0.01),while the apoptosis rate and the proportion of G0/G1 phase cells were significantly increased(all P<0.01).Compared with the si-LINC00519+anti-miR-NC group,the number of HGC-27 cell clones,the number of migrated and invaded cells,proportion of S-phase cells were significantly increased(all P<0.01),while the apoptosis rate and the proportion of G0/G1 phase cells in the si-LINC00519+anti-miR-876-3 p group were significantly decreased(all P<0.01).LINC00519 could target and negatively regulate miR-876-3 p expression.miR-876-3 p could target and negatively regulate HMGA1 expression.Conclusion:Knocking down LINC00519 could inhibit the proliferation,migration and invasion of gastric cancer HGC-27 cells and induce apoptosis by regulating the miR-876-3 p/HMGA1 axis.
作者
张丽柯
汪建光
景东帅
雷亮亮
刘德纯
ZHANG Like;WANG Jianguang;JING Dongshuai;LEI Liangliang;LIU Dechun(Department of Gastrointestinal Surgery,the First Affiliated Hospital of Henan University of Science and Technology,School of Clinical Medicine,Henan University of Science and Technology,Luoyang 471003,Henan,China)
出处
《中国肿瘤生物治疗杂志》
CAS
CSCD
北大核心
2021年第6期574-581,共8页
Chinese Journal of Cancer Biotherapy
