摘要
目的:采用网络药理学方法预测麻黄-白果药对治疗支气管哮喘(简称哮喘)和慢性阻塞性肺疾病(简称慢阻肺)的活性成分、靶点与信号通路,以探讨此药对“异病同治”的共同作用机制。方法:基于中药系统药理学数据库与分析平台获取麻黄-白果药对的活性化学成分及其对应靶点,通过Genecards数据库获取哮喘和慢阻肺相关靶点,将药对靶点与疾病靶点映射得到麻黄-白果药对作用于哮喘和慢阻肺的共同靶点。在Cytoscape软件中构建药对-活性成分-靶点-疾病网络,采用STRING在线数据库获取蛋白相互作用(PPI)数据。利用Bioconductor生物信息软件包对共同作用靶点进行KEGG通路富集分析。利用Auto Dock进行分子对接验证“异病同治”关键靶点和活性成分的相互作用。结果:通过筛选获得25个麻黄-白果药对的活性成分,108个麻黄-白果药对作用于哮喘和慢阻肺的共同靶点。富集分析结果表明麻黄-白果药对主要通过调节TNF信号通路、IL-17信号通路、HIF-1信号通路和Toll样受体信号通路等通路发挥对哮喘和慢阻肺的治疗作用。分子对接结果显示,“异病同治”关键靶点和活性成分之间具有较强的结合性。结论:麻黄-白果药对通过多成分、多靶点、多通路发挥了对哮喘和慢性阻塞性肺疾病“异病同治”的药效作用。
Objective:To predict the active components,targets and singal pathways of Ephedra and Ginkgo couplet medicine in the treatment of asthma and COPD by used the method of network pharmacology,so as to explore the common mechanism of“treating different diseases with the same treatment”.Methods:The effective chemical ingredients and corresponding targets of Ephedra and Ginkgo couplet medicine were obtained through TCMSP.Genecards was used to predict and screen the targets of asthma and COPD.The common targets of Ephedra and Ginkgo couplet medicine on asthma and COPD were obtained by mapping couplet medicine targets and disease targets.Couplet medicine-compound-target-disease network was constructed using Cytoscape software.Use STRING online database to obtain protein interaction(PPI)data.Bioconductor was used to analyze KEGG pathway enrichment of the targets.Use Auto Dock to carry out molecular docking to verify the interaction between active ingredients and key targets of“treating different diseases with the same treatment”.Results:25 chemical components of Ephedra and Ginkgo couplet medicine were obtained by database,108 potential targets of Ephedra and Ginkgo couplet medicine on asthma and COPD were obtained.The enrichment analysis results showed that Ephedra and Ginkgo couplet medicine mainly regulates TNF signaling pathway,IL-17 signaling pathway,HIF-1 signaling pathway,Toll-like receptor signaling pathway and other pathways to play a therapeutic effect on asthma and COPD.The results of molecular docking show that there is a strong binding between active ingredients and key targets of“treating different diseases with the same treatment”.Conclusion:Ephedra and Ginkgo couplet medicine exerts the effect of“treating different diseases with the same treatment”in asthma and COPD through multi-component,multi-target and multi-signal pathway.
作者
李竹英
陈璐
王丽洁
LI Zhuying;CHEN Lu;WANG Lijie(Department of Respiratory,the First Affiliated Hospital of Heilongjiang University of Chinese Medicine,Harbin 150040,China)
出处
《陕西中医》
CAS
2021年第8期1128-1132,共5页
Shaanxi Journal of Traditional Chinese Medicine
基金
国家自然科学基金资助面上项目(82074365)
黑龙江省自然科学基金资助面上项目(H2017068)
黑龙江中医药大学科研基金资助面上项目(201824)。
关键词
麻黄-白果
异病同治
哮喘
慢性阻塞性肺疾病
药理机制
Ephedra and Ginkgo
Treating different diseases with the same treatment
Asthma
Chronic obstructive pulmonary disease
Pharmacology mechanism
