摘要
目的建立分光光度法快速检测青方腐乳制品中硫化物(以Na_(2)S计)的方法。方法通过比较乙酸铅标准溶液反应前后的吸光度差值计算出消耗的乙酸铅量,换算得到样品中硫化钠的含量,以此间接检测方法判断样品中硫化物的添加情况。结果在干扰离子浓度为0.1 mol/L的条件下,经掩蔽剂去除后加标回收率均提高到88.45%~91.73%,相对标准偏差为0.28%~1.74%。乙酸铅分光光度检测结果在1~5 mg/kg范围内满足要求,相关系数大于0.999。测定硫化钠的方法定量限为6.0 mg/L。结论该方法操作简便快速,稳定性好、专属性强,仪器设备需求简单,适用于青方腐乳中非法使用硫化钠的快速检测。
Objective To establish a method for rapid determination of sulfide(based on Na_(2)S) in Qingfang sufu products by spectrophotometry. Methods The amount of lead acetate consumed was calculated by comparing the absorbance difference before and after the reaction of lead acetate standard solution, and the content of sodium sulfide in the sample was obtained by conversion. The addition of sulfide in the sample was determined by indirect detection method. Results Under the condition that the interfering ion concentration was 0.1 mol/L, the recovery rates of standard addition after the removal of the masking agent were increased to 88.45%-91.73%, and the relative standard deviations were 0.28%-1.74%. The results of lead acetate spectrophotometry met the requirements in the range of 1-5 mg/kg, and the correlation coefficient was greater than 0.999. The limit of quantitation of sodium sulfide was 6.0 mg/kg. Conclusion The method is simple, fast, and stable, and has strong specificity, and the demand of instrument and equipment is simple, which is suitable for rapid detection of the illegally used sodium sulfide in Qingfang sufu.
作者
李丰
毓志超
LI Feng;YU Zhi-Chao(Guangzhou Huibiao Testing Technology Center,Guangzhou 510670,China;Guangzhou Zhihui Biotechnology Co.,Ltd.,Guangzhou 510670,China)
出处
《食品安全质量检测学报》
CAS
北大核心
2021年第12期4845-4851,共7页
Journal of Food Safety and Quality
基金
广州市科技计划项目(201906010045)。
关键词
硫化钠
青方腐乳
分光光度法
快速检测
sodium sulfide
Qingfang sufu
spectrophotometry
rapid detection
