摘要
目的:观察脊神经后根切断兔膝关节软骨的变化特点,以及本体感受器和本体感觉通路的影响,探讨本体感觉障碍与膝骨关节炎间的联系。方法:按照随机分配原则将20只新西兰大白兔进行分组,对照组9只,模型组11只。模型组采用脊神经后根切断法离断L5-L6脊神经后根,对照组只切开皮肤及椎旁肌。术后8周应用改良Tarlov评分标准评估两组兔行为学差异,应用电生理-体感诱发电位(SEPs)和表面肌电图(EMG)评估本体感觉功能及肌群收缩情况,检测完成后统一处死行HE染色,应用Mankin′s评分评估两组膝关节软骨的病理情况,取右膝前交叉韧带(ACL)行Bielschowsky神经染色法观察两组韧带本体感受器的数量变化。结果:术后8周,模型组兔后肢活动呈轻度划步姿势,运动功能基本恢复正常;与对照组相比,行为学改变无明显差异(P>0.05);模型组兔SEPs和EMG与对照组相比,潜伏期延长,波幅下降,差异具有统计学意义(P<0.05),模型组兔关节软骨表面粗糙甚至消失,细胞排列紊乱等变化,Mankin′s评分显著高于对照组(P<0.01),软骨损伤更为严重;与对照组比较,模型组兔本体感受器数量明显减少,形态发生改变,韧带组织松散固缩,差异具有统计学意义(P<0.01);模型组兔软骨细胞过度凋亡,凋亡率明显高于对照组(P<0.01)。结论:脊神经后跟切断可导致本体感觉缺失,造成软骨细胞过度凋亡,关节软骨退变,促进膝骨关节炎的发生和发展。
Objective:To observe the characteristics of changes in the cartilage of the rabbit's knee joint after spinal nerve transection,as well as the influence of proprioceptors and proprioceptive pathways,and explore the relationship between proprio⁃ception disorder and knee osteoarthritis.Methods:According to the principle of random allocation,20 New Zealand white rabbits were divided into 2 groups,with 9 in the control group and 11 in the model group.In the model group,the posterior roots of the L5-L6 spinal nerve were cut off using the posterior root cutting method,while in the control group only the skin and paraspinal muscles were cut.Eight weeks after the operation,the modified Tarlov scoring standard was used to evaluate the behavioral differences be⁃tween the two groups and electrophysiology-Somatosensory Evoked Potentials(SEPs)and Surface Electromyography(EMG)were used to evaluate proprioception and muscle contraction.After the test was completed,they were sacrificed and HE staining⁃was performed.Mankin's score was used to evaluate the pathological conditions of the knee articular cartilage in the two groups.The anterior cruciate ligament(ACL)of the right knee was taken and the Bielschowsky nerve staining method was used to ob⁃serve the number of proprioceptors in the two groups.Results:Eight weeks after the operation,the hind limbs of the model group showed a light stroke posture,and the motor function basically returned to normal.Compared with the control group,there was no significant difference in behavioral changes(P>0.05);compared with the control group,the latencies of SEPs and EMG in the model group were longer and the amplitudes were lower(P<0.05).The rabbits’articular cartilage surface of the model group was rough or even disappeared,and the cell arrangement was disordered.The Mankin's score was significantly higher than that of the control group(P<0.01),and cartilage damage was more severe.Compared with the control group,the number of propriocep⁃tors in the model group was significantly reduced,the morphology changed,and the ligament tissue was loose and constricted(P<0.01);the rabbit chondrocytes in the model group were over-apopted and the rate of apoptosis was significantly higher than that of the control group(P<0.01).Conclusion:The transection of the spinal nerve can lead to loss of proprioception,excessive apop⁃tosis of chondrocytes,degeneration of articular cartilage,and promotion of the occurrence and development of knee osteoarthritis.
作者
胡荣庭
陈朝晖
王婷婷
HU Rong-ting;CHEN Zhao-hui;WANG Ting-ting(Graduate School,Anhui University of Chinese Medicine,Hefei 230038,China;College of Acupuncture and Massage,Anhui University of Chinese Medicine,Hefei 230038,China)
出处
《海南医学院学报》
CAS
2021年第16期1207-1212,共6页
Journal of Hainan Medical University
基金
安徽省教育厅自然基金重点项目(KJ2018A0273)
国家自然科学基金项目(8167151404)。
关键词
骨性关节炎
兔
本体感觉
软骨退变
细胞凋亡
Osteoarthritis
Rabbit
Proprioception
Cartilage degeneration
Apoptosis
