摘要
目的了解乙肝表面抗原(HBsAg)单试剂反应性献血者的复检情况以及分析相关影响因素。方法以在广州血液中心献血时血液筛查呈核酸检测(NAT)阴性、HBsAg ELISA检测单试剂反应性,且在献血6个月后回本中心进行复查的267名献血者为研究对象,分析其复查的ELISA和NAT结果;通过单因素分析和多因素逻辑回归分析,分析献血者年龄、性别、献血次数、职业以及教育程度与复查结果的关系。结果参加复检的267名献血者中,55名复检不合格(20.6%),其中HBsAg双试剂反应性15名,单试剂反应性33名,双试剂阴性7名(NAT阳性5名,ALT双试剂阳性2名)。单因素和多因素分析表明,影响复检结果的因素有年龄和献血次数:年龄越大,复检不合格率越高(Exp(B)=1.050,P=0.032);初次献血者的复检不合格率显著高于献血210次(Exp(B)=0.274,P<0.001)以及超过10次的献血者(Exp(B)=0.198,P=0.038)。结论 HBsAg单试剂反应性献血者复检不合格率较低,与献血者年龄和献血次数有关。本研究为固定献血者队伍的建设以及HBsAg单试剂反应性献血者的归队提供科学依据。
Objective To study the re-examination results of blood donors who were reactive for single ELISA assay of hepatitis B surface antigen(HBsAg)and analyze the related influencing factors. Methods A total of 267 blood donors from Guangzhou Blood Center were recruited. They were negative for nucleic acid testing(NAT)and single-reagent reactive for HBsAg ELISA test on their donation. They returned to our center for re-examination 6 months after donation. The NAT and ELISA results of re-examination were analyzed. The factors correlated with the re-examination results,including donors’ age,sex,blood donation times,occupation and education were analyzed by univariate analysis and multivariate logical regression analysis. Results Of the 267 donors,55(20.6%)were failure for the re-examination,including 15 cases of double-and 33 cases of single-reagent reactive for HBsAg,and 7 cases that were non-reactive for HBsAg(5 positive for NAT and 2 double-reagent reactive for ALT). Univariate and multivariate analysis showed that age and donation times were the factors independently associate with the re-examination results. Older age was associated with higher rate of re-examination failure(Exp(B)=1.050,P=0.032). The first-time blood donors had significantly higher rate of re-examination failure compared with those who donate 2-10 times(Exp(B)=0.274,P<0.001)and those who donated more than 10 times(Exp(B)=0.198,P=0.038). Conclusions The rate of re-examination failure in blood donors who were single-reagent reactive for HBsAg test was low,which was associated with age and donation times. This study provided scientific basis for the construction of regular blood donors and the reentry of blood donors who were single-reagent reactive for HBsAg.
作者
郑晓晓
许颖莹
柯蓉蓉
黎世杰
陈锦艳
ZHENG Xiao-xiao;XU Ying-ying;KE Rong-rong;LI Shi-jie;CHEN Jin-yan(Guangzhou Blood Center,the Key Medical Laboratory of Guangzhou,Guangzhou,Guangdong 510095,China)
出处
《热带医学杂志》
CAS
2021年第6期789-792,共4页
Journal of Tropical Medicine
基金
广东省医学科学技术研究基金项目(A2019204)
2019年中国输血协会威高科研基金(CSBT-WG-201906)
广州市医学重点学科建设项目(血液安全重点实验室)
广州市科技局基础与一般应用基础项目(202002030447)。
关键词
乙肝表面抗原
单试剂阳性
献血者归队
Hepatitis B surface antigen
Single reagent positive
Blood donors returned
