摘要
该研究以裂壶藻(Schizochytrium limacinum)渣为原料,采用酶解法制备得裂壶藻酶解物(S. limacinum hydrolysate, SLH),分析其美拉德反应条件,同时探究其不同超滤组分和葡聚糖凝胶柱层析组分美拉德反应产物(Maillard reaction products, MRPs)的抗氧化能力。结果表明,当还原糖为核糖、糖肽质量比为1∶1、反应pH为9、反应温度为100℃、反应时间为6 h时,SLH的MRPs还原力(5 mg·mL^(-1))为1.24,DPPH自由基清除率(12.5 mg·mL^(-1))为88.62%。将SLH经超滤后分别得到SLH-1 (<5 kD)、SLH-2 (<10 kD)和SLH-3 (<50 kD)组,发现SLH-1组在美拉德反应前后的抗氧化能力最强。将SLH-1组经Sephadex G-25凝胶柱分离后分别得到SLH-1-Ⅰ、SLH-1-Ⅱ、SLH-1-Ⅲ和SLH-1-Ⅳ组,发现分子量较大的SLH-1-Ⅰ组经美拉德反应修饰后的抗氧化活性最好,其还原力(2 mg·mL^(-1))为0.741,DPPH自由基的清除率(5 mg·mL^(-1))为79.41%。此外,氨基酸组成分析表明美拉德反应可导致酪氨酸(Tyr)、赖氨酸(Lys)、组氨酸(His)、精氨酸(Arg)、色氨酸(Trp)等氨基酸含量明显降低,但对必需氨基酸总含量影响不大。
The Schizochytrium limacinum hydrolysate(SLH) was prepared from defatted S. limacinum residues, so as to analyze its Maillard reaction conditions. Besides, we explored the antioxidant activities of Maillard reaction products(MRPs) from the isolated fractions of SLH by ultrafiltration and sephadex gel column chromatography. The optimal Maillard reaction parameters were obtained as follows: mass ratio(ribose: peptide) of 1∶1, pH of 9, temperature of 100 ℃ and time of 6 h(the reducing power activity was 1.24 at 5 mg·mL^(-1) and the DPPH radical scavenging rate was 88.62% at 12.5 mg·mL^(-1)). The SLH was ultra-filtered to obtain the fractions of SLH-1(<5 kD), SLH-2(<10 kD) and SLH-3(<50 kD). It is found that the SLH-1 had the strongest antioxidant capacity before and after Maillard reaction. Then the SLH-1 was further separated by Sephadex G-25 gel column to obtain the fractions of SLH-1-Ⅰ, SLH-1-Ⅱ, SLH-1-Ⅲ and SLH-1-Ⅳ. Higher molecular mass fraction of SLH-1-Ⅰ had higher antioxidant activity after Maillard reaction(the reducing power activity was 0.741 at 2 mg·mL-1 and the DPPH free radical scavenging rate was 79.41% at5 mg·mL^(-1)). Amino acid composition analysis shows that Maillard reaction could decrease the contents of Tyr, Lys, His, Arg and Trp, etc. However, there was no obvious change in the content of essential amino acids after Maillard reaction.
作者
胡晓
刘晶
高颖
李瑞杰
李来好
杨贤庆
陈胜军
吴燕燕
戚勃
荣辉
HU Xiao;LIU Jing;GAO Ying;LI Ruijie;LI Laihao;YANG Xianqing;CHEN Shengjun;WU Yanyan;QI Bo;RONG Hui(South China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences/Key Laboratory of Aquatic Product Pro-cessing,Ministry of Agriculture and Rural Affairs/National Research and Development Center for Aquatic Product Processing,Guangzhou 510300,China;College of Food Science and Engineering,Ocean University of China,Qingdao 266003,China)
出处
《南方水产科学》
CAS
CSCD
北大核心
2021年第4期58-65,共8页
South China Fisheries Science
基金
广东省重点领域研发计划资助项目(2020B1111030004)
财政部和农业农村部国家现代农业产业技术体系资助
广东省基础与应用基础研究基金项目(2019A1515011588)
中国水产科学研究院基本科研业务费项目(2020TD69)
中国水产科学研究院南海水产研究所中央级公益性科研院所基本科研业务费专项资金资助(2021SD06)。
关键词
裂壶藻
酶解物
美拉德反应
抗氧化活性
分离纯化
Schizochytrium limacinum
Hydrolysates
Maillard reaction
Antioxidant activity
Separation and purification
